p53 Protein (His tag)
-
- Target See all p53 (TP53) Proteins
- p53 (TP53) (Tumor Protein P53 (TP53))
- Protein Type
- Recombinant
- Biological Activity
- Active
-
Origin
- Human
-
Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This p53 protein is labelled with His tag.
- Application
- Standard (STD), Protein Interaction (PI), In vitro Assay (in vitro)
- Characteristics
- p53 is a tumor suppressor protein functioning as a sequence-specific transcription factor important for cell cycle arrest, apoptosis, and DNA-damage response. Recombinant p53, transcript variant 1, was expressed in E. coli from human cDNA (accession number NM_000546.5) with an N-terminal polyhistidine tag. Active Motif also offers Recombinant p53 protein (Catalog No. 31103) that was expressed in Baculovirus.
- Top Product
- Discover our top product TP53 Protein
-
Tumor Protein P53 (TP53) (AA 1-393) protein (GST tag)
TP53 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Tumor Protein P53 (TP53) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)TP53 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining STD, AbP
Tumor Protein P53 (TP53) (AA 1-391) protein (His tag)TP53 Origin: Rat Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Tumor Protein P53 (TP53) protein (His-SUMO Tag)TP53 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90% as determined by SDS-PAGE. ELISA
-
- Application Notes
- Recombinant p53 is suitable for use in Active Motif's TransAM® assay as a protein standard, in vitro transcription assays, protein-protein interactions and protein-DNA interaction studies. A recommended starting point for in vitro transcription assays is 20-100 ng per reaction. The molecular weight of the protein is 53 kDa.
- Restrictions
- For Research Use only
-
- Handling Advice
- Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
- Storage
- -80 °C
- Storage Comment
- Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation.
-
- Target
- p53 (TP53) (Tumor Protein P53 (TP53))
- Alternative Name
- p53 (TP53 Products)
- Background
- P53 is the most important tumor suppressor in the genome. It is responsive to numerous genotoxic stresses, which activates its transcription factor activity, in turn causing cell-cycle arrest by activating expression of p21 Cip/WAF. Mutant p53 that has lost its DNA-binding function interferes with the activity of native p53 and leads to oncogenic transformation. Alternatively, transformation may be caused by overexpression of Mdm2/Hdm2, an ubiquitin ligase specific for p53, which causes its destabilization. Inactivation of p53 is often coincident with hyperactivation of NFκB (NFκB p50 and NFκB p65), both of which serve to inhibit apoptosis.
- Pathways
- p53 Signaling, MAPK Signaling, PI3K-Akt Signaling, Apoptosis, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagy, Warburg Effect
-
