DGKA Protein (Transcript Variant 2) (Myc-DYKDDDDK Tag)
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- Target See all DGKA Proteins
- DGKA (Diacylglycerol Kinase, alpha 80kDa (DGKA))
- Protein Type
- Recombinant
- Protein Characteristics
- Transcript Variant 2
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This DGKA protein is labelled with Myc-DYKDDDDK Tag.
- Application
- Antibody Production (AbP), Standard (STD)
- Characteristics
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- Recombinant human DAG kinase alpha (transcript variant 2) protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
- Purity
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
- Top Product
- Discover our top product DGKA Protein
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Diacylglycerol Kinase, alpha 80kDa (DGKA) (AA 341-555) protein (His-IF2DI Tag)
DGKA Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Diacylglycerol Kinase, alpha 80kDa (DGKA) (AA 1-735) protein (GST tag)DGKA Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Diacylglycerol Kinase, alpha 80kDa (DGKA) (AA 1-735) protein (Strep Tag)Crystallography grade DGKA Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
Diacylglycerol Kinase, alpha 80kDa (DGKA) (AA 1-730) protein (Strep Tag)Crystallography grade DGKA Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
Diacylglycerol Kinase, alpha 80kDa (DGKA) proteinDGKA Origin: Mouse Host: Escherichia coli (E. coli) Recombinant > 95 % WB, SDS
Diacylglycerol Kinase, alpha 80kDa (DGKA) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)DGKA Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
Diacylglycerol Kinase, alpha 80kDa (DGKA) (Transcript Variant 4) protein (Myc-DYKDDDDK Tag)DGKA Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
Diacylglycerol Kinase, alpha 80kDa (DGKA) (Transcript Variant 3) protein (Myc-DYKDDDDK Tag)DGKA Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - Comment
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The tag is located at the C-terminal.
- Restrictions
- For Research Use only
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- Concentration
- 50 μg/mL
- Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Target
- DGKA (Diacylglycerol Kinase, alpha 80kDa (DGKA))
- Alternative Name
- Dag Kinase alpha (DGKA Products)
- Synonyms
- DAGK Protein, DAGK1 Protein, DGK-alpha Protein, 80kDa Protein, AW146112 Protein, Dagk1 Protein, dgka Protein, zgc:136759 Protein, dagk Protein, dagk1 Protein, dgk-alpha Protein, diacylglycerol kinase alpha Protein, diacylglycerol kinase, alpha Protein, diacylglycerol kinase, alpha 80kDa Protein, diacylglycerol kinase, alpha a Protein, diacylglycerol kinase alpha S homeolog Protein, DGKA Protein, Dgka Protein, dgkaa Protein, Tsp_02164 Protein, dgka Protein, dgka.S Protein
- Background
- The protein encoded by this gene belongs to the eukaryotic diacylglycerol kinase family. It acts as a modulator that competes with protein kinase C for the second messenger diacylglycerol in intracellular signaling pathways. It also plays an important role in the resynthesis of phosphatidylinositols and phosphorylating diacylglycerol to phosphatidic acid. Alternative splicing occurs at this locus and four transcript variants encoding the same protein have been identified.
- Molecular Weight
- 82.5 kDa
- NCBI Accession
- NP_958853
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