FGF2 Protein
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- Target See all FGF2 Proteins
- FGF2 (Fibroblast Growth Factor 2 (Basic) (FGF2))
- Protein Type
- Recombinant
- Biological Activity
- Active
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Application
- Functional Studies (Func), Antibody Production (AbP), Standard (STD), Protein Interaction (PI)
- Specificity
- Optimal preservation of protein structure, post-translational modifications and functions.
- Characteristics
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- Recombinant human FGF basic / FGF2 protein expressed in E. coli.
- Produced with end-sequenced ORF clone
- Tested for bioactivity.
- Purity
- Purity is > 98 % , as determined by Coomassie stained SDS-PAGE.
- Endotoxin Level
- Less than 0.01 ng per ug protein as determined by the LAL method.
- Biological Activity Comment
- The ED50 is 1 - 4 ng/ml, corresponding to a specific activity of 1 - 0.25 x 10^6 units/mg, determined by the dose dependent stimulation of NIH/ 3T3 cell proliferation. The bioactivity is equivalent to competitor reported values.
- Top Product
- Discover our top product FGF2 Protein
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays - Restrictions
- For Research Use only
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- Concentration
- > 50 μg/mL
- Buffer
- 10 mM NaH2PO4, 150 mM NaCl, pH 7.2, 1 mM DTT
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Target
- FGF2 (Fibroblast Growth Factor 2 (Basic) (FGF2))
- Alternative Name
- Fgf Basic,fgf2 (FGF2 Products)
- Synonyms
- BFGF Protein, FGF-2 Protein, FGFB Protein, HBGF-2 Protein, Fgf-2 Protein, Fgfb Protein, bFGF Protein, fibroblast growth factor 2 Protein, fibroblast growth factor 2 (basic) Protein, FGF2 Protein, Fgf2 Protein, fgf2 Protein
- Background
- The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. This protein has been implicated in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. The mRNA for this gene contains multiple polyadenylation sites, and is alternatively translated from non-AUG (CUG) and AUG initiation codons, resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF.
- Molecular Weight
- 16.3 kDa
- NCBI Accession
- NP_001997
- Pathways
- RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, C21-Steroid Hormone Metabolic Process, Inositol Metabolic Process, Glycosaminoglycan Metabolic Process, Protein targeting to Nucleus, S100 Proteins
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