RASGRF1 Protein (Transcript Variant 1) (Myc-DYKDDDDK Tag)

Catalog No. ABIN2712357

Product Details

Target: RASGRF1 (Ras Protein-Specific Guanine Nucleotide-Releasing Factor 1 (RASGRF1))

Protein Type: Recombinant

Protein Characteristics: Transcript Variant 1

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This RASGRF1 protein is labelled with Myc-DYKDDDDK Tag.

Application: Antibody Production (AbP), Standard (STD)

Characteristics:

• Recombinant human RASGRF1 (transcript variant 1) protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: RASGRF1 (Ras Protein-Specific Guanine Nucleotide-Releasing Factor 1 (RASGRF1))

Alternative Name: Rasgrf1 (RASGRF1 Products)

Synonyms: CDC25 Protein, CDC25L Protein, GNRP Protein, GRF1 Protein, GRF55 Protein, H-GRF55 Protein, PP13187 Protein, ras-GRF1 Protein, AI844718 Protein, CDC25Mm Protein, Grf1 Protein, Grfbeta Protein, P190-A Protein, Ras-GRF1 Protein, p190 Protein, p190RhoGEF Protein, cdc25 Protein, rasgrf1 Protein, Ras protein specific guanine nucleotide releasing factor 1 Protein, RAS protein-specific guanine nucleotide-releasing factor 1 Protein, Ras protein specific guanine nucleotide releasing factor 1 S homeolog Protein, si:ch211-234p18.3 Protein, RASGRF1 Protein, Rasgrf1 Protein, rasgrf1.S Protein, si:ch211-234p18.3 Protein, rasgrf1 Protein

Background: The protein encoded by this gene is a guanine nucleotide exchange factor (GEF) similar to the Saccharomyces cerevisiae CDC25 gene product. Functional analysis has demonstrated that this protein stimulates the dissociation of GDP from RAS protein. The studies of the similar gene in mouse suggested that the Ras-GEF activity of this protein in brain can be activated by Ca2+ influx, muscarinic receptors, and G protein beta-gamma subunit. Mouse studies also indicated that the Ras-GEF signaling pathway mediated by this protein may be important for long-term memory. Alternatively spliced transcript variants encoding distinct isoforms have been reported.

Molecular Weight: 145.1 kDa

NCBI Accession: NP_002882

Pathways: RTK Signaling, Cell Division Cycle, M Phase