Cytokeratin 18 Protein (His tag)

Catalog No. ABIN2712825

Product Details

Target: Cytokeratin 18 (KRT18) (Keratin 18 (KRT18))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This Cytokeratin 18 protein is labelled with His tag.

Application: Antibody Production (AbP), Standard (STD)

Characteristics:

• Recombinant human Cytokeratin 18 (full length, N-term HIS tag, transcript variant 1) protein expressed in E. coli.
• Produced with end-sequenced ORF clone

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the N-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris, pH 8.0, 150 mM NaCl, 10 % glycerol, 1 % Sarkosyl. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: Cytokeratin 18 (KRT18) (Keratin 18 (KRT18))

Alternative Name: Cytokeratin 18 (KRT18 Products)

Synonyms: CK18 Protein, K18 Protein, Krt1-18 Protein, CYK18 Protein, DreK18 Protein, cb83 Protein, dapk1 Protein, sb:cb83 Protein, wu:fa13f02 Protein, wu:fb36b04 Protein, krt18 Protein, MGC64569 Protein, MGC75922 Protein, KRT18 Protein, CK-18-A Protein, K18-A Protein, NC-11 Protein, cyk18 Protein, k18 Protein, krt18-a Protein, krt18-b Protein, krt18a Protein, xkendob Protein, keratin 18 Protein, keratin 18, type I L homeolog Protein, keratin 18, type I Protein, keratin, type I cytoskeletal 18 Protein, K18, simple type I keratin Protein, Keratin, type I cytoskeletal 18 Protein, keratin 18, type I S homeolog Protein, Krt18 Protein, KRT18 Protein, krt18 Protein, krt18.L Protein, LOC700569 Protein, LOC100136778 Protein, k1c18 Protein, LOC100008885 Protein, krt18.S Protein

Background: KRT18 encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene.

Molecular Weight: 47.9 kDa

NCBI Accession: NP_000215

Pathways: Apoptosis, Caspase Cascade in Apoptosis