CBFB Protein (Transcript Variant 2) (Myc-DYKDDDDK Tag)

Catalog No. ABIN2713253

Product Details

Target: CBFB (Core-Binding Factor, beta Subunit (CBFB))

Protein Type: Recombinant

Protein Characteristics: Transcript Variant 2

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This CBFB protein is labelled with Myc-DYKDDDDK Tag.

Application: Antibody Production (AbP), Standard (STD)

Characteristics:

• Recombinant human CBFB (transcript variant 2) protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: CBFB (Core-Binding Factor, beta Subunit (CBFB))

Alternative Name: Cbfb (CBFB Products)

Synonyms: PEBP2B Protein, Cbfb Protein, pebp2b Protein, Pebp2 Protein, CBFB Protein, unp1266 Protein, cbf-beta Protein, cbfbeta Protein, pea2-beta Protein, pebp2-beta Protein, AI893578 Protein, PEA2 Protein, PEBP2b Protein, Pebpb2 Protein, core-binding factor beta subunit Protein, core binding factor beta subunit Protein, core-binding factor, beta subunit Protein, core-binding factor, beta subunit S homeolog Protein, core binding factor beta Protein, CBFB Protein, Cbfb Protein, cbfb Protein, cbfb.S Protein

Background: The protein encoded by this gene is the beta subunit of a heterodimeric core-binding transcription factor belonging to the PEBP2/CBF transcription factor family which master-regulates a host of genes specific to hematopoiesis (e.g., RUNX1) and osteogenesis (e.g., RUNX2). The beta subunit is a non-DNA binding regulatory subunit it allosterically enhances DNA binding by alpha subunit as the complex binds to the core site of various enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers and GM-CSF promoters. Alternative splicing generates two mRNA variants, each encoding a distinct carboxyl terminus. In some cases, a pericentric inversion of chromosome 16 [inv(16)(p13q22)] produces a chimeric transcript consisting of the N terminus of core-binding factor beta in a fusion with the C-terminal portion of the smooth muscle myosin heavy chain 11. This chromosomal rearrangement is associated with acute myeloid leukemia of the M4Eo subtype. Two transcript variants encoding different isoforms have been found for this gene.

Molecular Weight: 21.3 kDa

NCBI Accession: NP_001746

Pathways: Regulation of Lipid Metabolism by PPARalpha