DARC Protein (Transcript Variant 2) (Myc-DYKDDDDK Tag)
-
- Target See all DARC Proteins
- DARC (Duffy Blood Group, Chemokine Receptor (DARC))
- Protein Type
- Recombinant
- Protein Characteristics
- Transcript Variant 2
-
Origin
- Human
-
Source
- HEK-293 Cells
- Purification tag / Conjugate
- This DARC protein is labelled with Myc-DYKDDDDK Tag.
- Application
- Antibody Production (AbP), Standard (STD)
- Characteristics
-
- Recombinant human CD234 / DARC (transcript variant 2) protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
- Purity
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
- Top Product
- Discover our top product DARC Protein
-
Duffy Blood Group, Chemokine Receptor (DARC) (AA 1-336) protein (GST tag)
DARC Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Duffy Blood Group, Chemokine Receptor (DARC) (AA 1-336) protein (Strep Tag)Crystallography grade DARC Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
Duffy Blood Group, Chemokine Receptor (DARC) (AA 1-334) protein (Strep Tag)Crystallography grade DARC Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
Duffy Blood Group, Chemokine Receptor (DARC) proteinDARC Origin: Mouse Host: Escherichia coli (E. coli) Recombinant > 95 % WB, SDS, ELISA, IP
-
- Application Notes
-
Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - Comment
-
The tag is located at the C-terminal.
- Restrictions
- For Research Use only
-
- Concentration
- 50 μg/mL
- Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
-
- Target
- DARC (Duffy Blood Group, Chemokine Receptor (DARC))
- Alternative Name
- Cd234,darc (DARC Products)
- Synonyms
- DARC Protein, FY Protein, ACKR1 Protein, CCBP1 Protein, CD234 Protein, Dfy Protein, GPD Protein, GpFy Protein, WBCQ1 Protein, AA162249 Protein, Ackr1 Protein, ESTM35 Protein, Duffy blood group, chemokine receptor Protein, atypical chemokine receptor 1 (Duffy blood group) Protein, atypical chemokine receptor 1 Protein, DARC Protein, ACKR1 Protein, Ackr1 Protein
- Background
- Atypical chemokine receptor that controls chemokine levels and localization via high-affinity chemokine binding that is uncoupled from classic ligand-driven signal transduction cascades, resulting instead in chemokine sequestration, degradation, or transcytosis. Also known as interceptor (internalizing receptor) or chemokine-scavenging receptor or chemokine decoy receptor. Has a promiscuous chemokine-binding profile, interacting with inflammatory chemokines of both the CXC and the CC subfamilies but not with homeostatic chemokines. Acts as a receptor for chemokines including CCL2, CCL5, CCL7, CCL11, CCL13, CCL14, CCL17, CXCL5, CXCL6, IL8/CXCL8, CXCL11, GRO, RANTES, MCP-1, TARC and also for the malaria parasites P.vivax and P.knowlesi. May regulate chemokine bioavailability and, consequently, leukocyte recruitment through two distinct mechanisms: when expressed in endothelial cells, it sustains the abluminal to luminal transcytosis of tissue-derived chemokines and their subsequent presentation to circulating leukocytes when expressed in erythrocytes, serves as blood reservoir of cognate chemokines but also as a chemokine sink, buffering potential surges in plasma chemokine levels. [UniProtKB/Swiss-Prot Function]
- Molecular Weight
- 35.4 kDa
- NCBI Accession
- NP_002027
-
