TNFRSF11A Protein
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- Target See all TNFRSF11A Proteins
- TNFRSF11A (Tumor Necrosis Factor Receptor Superfamily, Member 11a, NFKB Activator (TNFRSF11A))
- Protein Type
- Recombinant
- Biological Activity
- Active
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Application
- Functional Studies (Func), Protein Interaction (PI), Standard (STD), Antibody Production (AbP)
- Specificity
- Optimal preservation of protein structure, post-translational modifications and functions.
- Characteristics
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- Recombinant human CD265 / RANK protein expressed in E. coli.
- Produced with end-sequenced ORF clone
- Tested for bioactivity.
- Purity
- > 95 % as determined by SDS-PAGE and Coomassie blue staining
- Endotoxin Level
- Endotoxin level is <0.1 ng/μg of protein (<1EU/μg).
- Biological Activity Comment
- Determined by its ability to inhibit sRANKL induced NFkB in RAW264.7 cells in the absence of any cross-linking. The expected ED50 for this effect in the presence of 15ng/ml of recombinant sRANKL, is 30-50 ng/ml.
- Top Product
- Discover our top product TNFRSF11A Protein
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays - Restrictions
- For Research Use only
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- Buffer
- Lyophilized from a 0.2 μM filtered solution of 20 mM phosphate buffer,100 mM NaCl, pH 7.2
- Handling Advice
- Resuspend the protein in the desired concentration in proper buffer
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Target
- TNFRSF11A (Tumor Necrosis Factor Receptor Superfamily, Member 11a, NFKB Activator (TNFRSF11A))
- Alternative Name
- Cd265,rank (TNFRSF11A Products)
- Synonyms
- RANK Protein, RGD1563614 Protein, TNFRSF11A Protein, Ly109 Protein, ODFR Protein, OFE Protein, Rank Protein, TRANCE-R Protein, mRANK Protein, CD265 Protein, FEO Protein, LOH18CR1 Protein, OPTB7 Protein, OSTS Protein, PDB2 Protein, TRANCER Protein, TNF receptor superfamily member 11A Protein, TNF receptor superfamily member 11a Protein, tumor necrosis factor receptor superfamily, member 11a, NFKB activator Protein, Tnfrsf11a Protein, TNFRSF11A Protein
- Background
- The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptors can interact with various TRAF family proteins, through which this receptor induces the activation of NF-kappa B and MAPK8/JNK. This receptor and its ligand are important regulators of the interaction between T cells and dendritic cells. This receptor is also an essential mediator for osteoclast and lymph node development. Mutations at this locus have been associated with familial expansile osteolysis, autosomal recessive osteopetrosis, and Paget disease of bone. Alternatively spliced transcript variants have been described for this locus.
- Molecular Weight
- 19.3 kDa
- NCBI Accession
- NP_003830
- Pathways
- NF-kappaB Signaling
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