CD73 Protein (Transcript Variant 1) (His tag)

Catalog No. ABIN2713773

Product Details

Target: CD73 (NT5E) (5'-Nucleotidase, Ecto (CD73) (NT5E))

Protein Type: Recombinant

Protein Characteristics: Transcript Variant 1

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This CD73 protein is labelled with His tag.

Application: Antibody Production (AbP), Standard (STD)

Characteristics:

• Recombinant human CD73 (transcript variant 1) protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Purity: > 95 % as determined by SDS-PAGE and Coomassie blue staining

Endotoxin Level: Endotoxin level is <0.1 ng/μg of protein (<1EU/μg).

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Buffer: Supplied as a 0.2 μM filtered solution of 20 mM Tris-HCl, 150 mM NaCl, 2 mM CaCl2, 10 % Glycerol, pH 8.0

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: CD73 (NT5E) (5'-Nucleotidase, Ecto (CD73) (NT5E))

Alternative Name: CD73 (NT5E Products)

Synonyms: CALJA Protein, CD73 Protein, E5NT Protein, NT Protein, NT5 Protein, NTE Protein, eN Protein, eNT Protein, zgc:63784 Protein, NT5E Protein, MGC114869 Protein, PSPTO2277 Protein, 2210401F01Rik Protein, AI447961 Protein, Nt5 Protein, 5'-nucleotidase ecto Protein, 5'-nucleotidase, ecto (CD73) Protein, 5'-nucleotidase Protein, 5' nucleotidase, ecto Protein, NT5E Protein, nt5e Protein, nt5e.S Protein, PSPTO_2277 Protein, CBU_1233 Protein, MCA1322 Protein, Nt5e Protein

Background: The protein encoded by this gene is a plasma membrane protein that catalyzes the conversion of extracellular nucleotides to membrane-permeable nucleosides. The encoded protein is used as a determinant of lymphocyte differentiation. Defects in this gene can lead to the calcification of joints and arteries. Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2011]

Molecular Weight: 58.76kD

NCBI Accession: NP_002517

Pathways: Synaptic Membrane, Ribonucleoside Biosynthetic Process