CD81 Protein (CD81) (Myc-DYKDDDDK Tag)

Catalog No. ABIN2713788

Product Details

Target: CD81

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This CD81 protein is labelled with Myc-DYKDDDDK Tag.

Application: Antibody Production (AbP), Standard (STD)

Characteristics:

• Recombinant human CD81 / TAPA1 protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

References

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Winston, Goetzl, Akers, Carter, Rockenstein, Galasko, Masliah, Rissman: "Prediction of conversion from mild cognitive impairment to dementia with neuronally derived blood exosome protein profile." in: Alzheimer's & dementia (Amsterdam, Netherlands), Vol. 3, pp. 63-72, (2016) (PubMed).

Kapogiannis, Boxer, Schwartz, Abner, Biragyn, Masharani, Frassetto, Petersen, Miller, Goetzl: "Dysfunctionally phosphorylated type 1 insulin receptor substrate in neural-derived blood exosomes of preclinical Alzheimer's disease." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 29, Issue 2, pp. 589-96, (2015) (PubMed).

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Fiandaca, Kapogiannis, Mapstone, Boxer, Eitan, Schwartz, Abner, Petersen, Federoff, Miller, Goetzl: "Identification of preclinical Alzheimer's disease by a profile of pathogenic proteins in neurally derived blood exosomes: A case-control study." in: Alzheimer's & dementia : the journal of the Alzheimer's Association, (2014) (PubMed).

Myers, Maxwell, Wang: "Quantification of XRCC and DNA-PK proteins in cancer cell lines and human tumors by LC-MS/MS." in: Bioanalysis, Vol. 6, Issue 22, pp. 2969-83, (2014) (PubMed).

Lawrence, Conderino, Rieder: "Redistribution of demethylated RNA helicase A during foot-and-mouth disease virus infection: role of Jumonji C-domain containing protein 6 in RHA demethylation." in: Virology, Vol. 452-453, pp. 1-11, (2014) (PubMed).

Bando, Iguchi, Fukuoka, Yamamoto, Hidaka-Takeno, Okimura, Matsumoto, Suda, Nishizawa, Takahashi, Tojo, Takahashi: "Involvement of PIT-1-reactive cytotoxic T lymphocytes in anti-PIT-1 antibody syndrome." in: The Journal of clinical endocrinology and metabolism, Vol. 99, Issue 9, pp. E1744-9, (2014) (PubMed).

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Target Details

Target: CD81

Alternative Name: Cd81,tapa1 (CD81 Products)

Synonyms: Tapa-1 Protein, Tapa1 Protein, Tspan28 Protein, CVID6 Protein, S5.7 Protein, TAPA1 Protein, TSPAN28 Protein, CD81 molecule Protein, CD81 molecule b Protein, CD81 antigen Protein, Cd81 molecule Protein, CD81 Protein, cd81b Protein, Cd81 Protein

Background: The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein that is known to complex with integrins. This protein appears to promote muscle cell fusion and support myotube maintenance. Also it may be involved in signal transduction. This gene is localized in the tumor-suppressor gene region and thus it is a candidate gene for malignancies. Two transcript variants encoding different isoforms have been found for this gene.

Molecular Weight: 25.6 kDa

NCBI Accession: NP_004347

Pathways: Inositol Metabolic Process, Hepatitis C