Chitotriosidase 1 Protein (His tag)
-
- Target See all Chitotriosidase 1 (CHIT1) Proteins
- Chitotriosidase 1 (CHIT1) (Chitotriosidase (CHIT1))
- Protein Type
- Recombinant
-
Origin
- Human
-
Source
- HEK-293 Cells
- Purification tag / Conjugate
- This Chitotriosidase 1 protein is labelled with His tag.
- Application
- Antibody Production (AbP), Standard (STD)
- Characteristics
-
- Recombinant human Chitinase 1 protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
- Purity
- > 95 % as determined by SDS-PAGE and Coomassie blue staining
- Endotoxin Level
- < 0.1 EU per μg protein as determined by LAL test
- Top Product
- Discover our top product CHIT1 Protein
-
Chitotriosidase (CHIT1) (AA 1-466) protein (GST tag)
CHIT1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Chitotriosidase (CHIT1) (AA 60-169) protein (GST tag)CHIT1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Chitotriosidase (CHIT1) (AA 22-466) protein (His tag)Crystallography grade CHIT1 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Chitotriosidase (CHIT1) (AA 22-464) protein (His tag)Crystallography grade CHIT1 Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Chitotriosidase (CHIT1) protein (Myc-DYKDDDDK Tag)CHIT1 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
-
- Application Notes
-
Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - Comment
-
The tag is located at the N-terminal.
- Restrictions
- For Research Use only
-
- Concentration
- 50 μg/mL
- Buffer
- 20 mM Tris-HCl, 150 mM NaCl, pH 7.5. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
-
- Target
- Chitotriosidase 1 (CHIT1) (Chitotriosidase (CHIT1))
- Alternative Name
- Chitinase 1 (CHIT1 Products)
- Synonyms
- CHI3 Protein, CHIT Protein, CHITD Protein, CHIT1 Protein, chio Protein, 2300002L19Rik Protein, chitinase 1 Protein, chitinase 1 (chitotriosidase) Protein, CHIT1 Protein, chit1 Protein, Chit1 Protein
- Background
- Chitotriosidase is secreted by activated human macrophages and is markedly elevated in plasma of Gaucher disease patients. The expression of chitotriosidase occurs only at a late stage of differentiation of monocytes to activated macrophages in culture. Human macrophages can synthesize a functional chitotriosidase, a highly conserved enzyme with a strongly regulated expression. This enzyme may play a role in the degradation of chitin-containing pathogens. Several alternatively spliced transcript variants have been described for this gene.
- Molecular Weight
- 50.4 kDa
- NCBI Accession
- NP_003456
-
