TNFSF9 Protein

Catalog No. ABIN2714235

Product Details

Target: TNFSF9 (Tumor Necrosis Factor (Ligand) Superfamily, Member 9 (TNFSF9))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Application: Functional Studies (Func), Protein Interaction (PI), Antibody Production (AbP), Standard (STD)

Specificity: Optimal preservation of protein structure, post-translational modifications and functions.

Characteristics:

• Recombinant human 4-1BBL / TNFSF9 protein expressed in E. coli.
• Produced with end-sequenced ORF clone
• Tested for bioactivity.

Purity: > 95 % as determined by SDS-PAGE and Coomassie blue staining

Endotoxin Level: Endotoxin level is <0.1 ng/μg of protein (<1EU/μg).

Biological Activity Comment: Determined by the dose-dependent stimulation of IL-8 production by human PBMC. ED50 for this effect is 5-10 ng/ml.Please Note: Results may vary with PBMC donors.

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays

Restrictions: For Research Use only

Handling

Buffer: Lyophilized from a 0.2 μM filtered solution of 20 mM phosphate buffer,100 mM NaCl, pH 7.2

Handling Advice: Resuspend the protein in the desired concentration in proper buffer

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: TNFSF9 (Tumor Necrosis Factor (Ligand) Superfamily, Member 9 (TNFSF9))

Alternative Name: 4-1bbl,tnfsf9 (TNFSF9 Products)

Synonyms: TNFSF9 Protein, 4-1BB-L Protein, 4-1BBL Protein, AI848817 Protein, Cd137l Protein, Ly63l Protein, CD137L Protein, TNF superfamily member 9 Protein, tumor necrosis factor (ligand) superfamily, member 9 Protein, Tnfsf9 Protein, LOC476729 Protein, TNFSF9 Protein

Background: The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This transmembrane cytokine is a bidirectional signal transducer that acts as a ligand for TNFRSF9/4-1BB, which is a costimulatory receptor molecule in T lymphocytes. This cytokine and its receptor are involved in the antigen presentation process and in the generation of cytotoxic T cells. The receptor TNFRSF9/4-1BB is absent from resting T lymphocytes but rapidly expressed upon antigenic stimulation. The ligand encoded by this gene, TNFSF9/4-1BBL, has been shown to reactivate anergic T lymphocytes in addition to promoting T lymphocyte proliferation. This cytokine has also been shown to be required for the optimal CD8 responses in CD8 T cells. This cytokine is expressed in carcinoma cell lines, and is thought to be involved in T cell-tumor cell interaction.[provided by RefSeq, Oct 2008].

Molecular Weight: 19.5 kDa

NCBI Accession: NP_003802

Pathways: Activated T Cell Proliferation, Cancer Immune Checkpoints