ASAH1 Protein (Transcript Variant 2) (Myc-DYKDDDDK Tag)

Catalog No. ABIN2714319

Product Details

Target: ASAH1 (N-Acylsphingosine Amidohydrolase (Acid Ceramidase) 1 (ASAH1))

Protein Type: Recombinant

Protein Characteristics: Transcript Variant 2

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This ASAH1 protein is labelled with Myc-DYKDDDDK Tag.

Application: Antibody Production (AbP), Standard (STD)

Characteristics:

• Recombinant human Acid ceramidase (transcript variant 2) protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: ASAH1 (N-Acylsphingosine Amidohydrolase (Acid Ceramidase) 1 (ASAH1))

Alternative Name: Acid Ceramidase (ASAH1 Products)

Synonyms: AC Protein, ACDase Protein, ASAH Protein, PHP Protein, PHP32 Protein, SMAPME Protein, 2310081N20Rik Protein, Asah Protein, MGC82286 Protein, asah1 Protein, zgc:101637 Protein, ASAH1 Protein, zgc:66026 Protein, N-acylsphingosine amidohydrolase 1 Protein, N-acylsphingosine amidohydrolase (acid ceramidase) 1 Protein, N-acylsphingosine amidohydrolase (acid ceramidase) 1 L homeolog Protein, N-acylsphingosine amidohydrolase (acid ceramidase) 1a Protein, Acid ceramidase Protein, N-acylsphingosine amidohydrolase (acid ceramidase) 1b Protein, ASAH1 Protein, Asah1 Protein, asah1.L Protein, asah1a Protein, asah-1 Protein, asah1b Protein

Background: This gene encodes a member of the acid ceramidase family of proteins. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed. Processing of this preproprotein generates alpha and beta subunits that heterodimerize to form the mature lysosomal enzyme, which catalyzes the degradation of ceramide into sphingosine and free fatty acid. This enzyme is overexpressed in multiple human cancers and may play a role in cancer progression. Mutations in this gene are associated with the lysosomal storage disorder, Farber lipogranulomatosis, and a neuromuscular disorder, spinal muscular atrophy with progressive myoclonic epilepsy.

Molecular Weight: 46.3 kDa

NCBI Accession: NP_004306