PRKAB1 Protein (Myc-DYKDDDDK Tag)
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- Target See all PRKAB1 Proteins
- PRKAB1 (Protein Kinase, AMP-Activated, beta 1 Non-Catalytic Subunit (PRKAB1))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This PRKAB1 protein is labelled with Myc-DYKDDDDK Tag.
- Application
- Antibody Production (AbP), Standard (STD)
- Characteristics
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- Recombinant human AMPK beta-1 chain / AMPKb protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
- Purity
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
- Top Product
- Discover our top product PRKAB1 Protein
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Protein Kinase, AMP-Activated, beta 1 Non-Catalytic Subunit (PRKAB1) (AA 62-269) protein (His-IF2DI Tag)
PRKAB1 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Protein Kinase, AMP-Activated, beta 1 Non-Catalytic Subunit (PRKAB1) (AA 1-270) protein (GST tag)PRKAB1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Protein Kinase, AMP-Activated, beta 1 Non-Catalytic Subunit (PRKAB1) (AA 2-270) protein (His tag)Crystallography grade PRKAB1 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Protein Kinase, AMP-Activated, beta 1 Non-Catalytic Subunit (PRKAB1) (AA 2-270) protein (His tag)Crystallography grade PRKAB1 Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Protein Kinase, AMP-Activated, beta 1 Non-Catalytic Subunit (PRKAB1) protein (His tag)PRKAB1 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 85 % by SDS - PAGE SDS
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - Comment
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The tag is located at the C-terminal.
- Restrictions
- For Research Use only
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- Concentration
- 50 μg/mL
- Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Target
- PRKAB1 (Protein Kinase, AMP-Activated, beta 1 Non-Catalytic Subunit (PRKAB1))
- Alternative Name
- Ampk beta-1 Chain,ampkb (PRKAB1 Products)
- Background
- Non-catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton probably by indirectly activating myosin. Beta non-catalytic subunit acts as a scaffold on which the AMPK complex assembles, via its C-terminus that bridges alpha (PRKAA1 or PRKAA2) and gamma subunits (PRKAG1, PRKAG2 or PRKAG3). [UniProtKB/Swiss-Prot Function]
- Molecular Weight
- 30.2 kDa
- NCBI Accession
- NP_006244
- Pathways
- AMPK Signaling, Warburg Effect
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