DDX39B Protein (Transcript Variant 1) (Myc-DYKDDDDK Tag)
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- Target See all DDX39B Proteins
- DDX39B (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 39B (DDX39B))
- Protein Type
- Recombinant
- Protein Characteristics
- Transcript Variant 1
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This DDX39B protein is labelled with Myc-DYKDDDDK Tag.
- Application
- Antibody Production (AbP), Standard (STD)
- Characteristics
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- Recombinant human BAT1 / UAP56 (transcript variant 1) protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
- Purity
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
- Top Product
- Discover our top product DDX39B Protein
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DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 39B (DDX39B) (AA 1-428) protein (His tag)
DDX39B Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 39B (DDX39B) (AA 2-428) protein (His tag)Crystallography grade DDX39B Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, SDS, WB, Crys
DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 39B (DDX39B) (AA 2-428) protein (His tag)Crystallography grade DDX39B Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, SDS, WB, Crys
DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 39B (DDX39B) (AA 2-251), (partial) protein (GST tag)DDX39B Origin: Human Host: Escherichia coli (E. coli) Recombinant 90 % ELISA, SDS
DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 39B (DDX39B) (Transcript Variant 2) protein (Myc-DYKDDDDK Tag)DDX39B Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - Comment
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The tag is located at the C-terminal.
- Restrictions
- For Research Use only
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- Concentration
- 50 μg/mL
- Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Target
- DDX39B (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 39B (DDX39B))
- Alternative Name
- Bat1,uap56 (DDX39B Products)
- Synonyms
- BAT1 Protein, D6S81E Protein, UAP56 Protein, Bat1 Protein, Bat1a Protein, p47 Protein, 0610030D10Rik Protein, AI428441 Protein, Bat-1 Protein, D17H6S81E Protein, D17H6S81E-1 Protein, D6S81Eh Protein, bat1 Protein, zgc:63773 Protein, DExD-box helicase 39B Protein, DEAD (Asp-Glu-Ala-Asp) box polypeptide 39B Protein, DDX39B Protein, Ddx39b Protein, ddx39b Protein
- Background
- Splice factor that is required for the first ATP-dependent step in spliceosome assembly and for the interaction of U2 snRNP with the branchpoint. Has both RNA-stimulated ATP binding/hydrolysis activity and ATP-dependent RNA unwinding activity. Even with the stimulation of RNA, the ATPase activity is weak. Can only hydrolyze ATP but not other NTPs. The RNA stimulation of ATPase activity does not have a strong preference for the sequence and length of the RNA. However, ssRNA stimulates the ATPase activity much more strongly than dsRNA. Can unwind 5' or 3' overhangs or blunt end RNA duplexes in vitro. The ATPase and helicase activities are not influenced by U2AF2 the effect of ALYREF/THOC4 is reported conflictingly with [PubMed:23299939] reporting a stimulatory effect. [UniProtKB/Swiss-Prot Function]
- Molecular Weight
- 48.8 kDa
- NCBI Accession
- NP_004631
- Pathways
- Ribonucleoprotein Complex Subunit Organization
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