BAD Protein (His tag)
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- Target See all BAD Proteins
- BAD (BCL2-Associated Agonist of Cell Death (BAD))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This BAD protein is labelled with His tag.
- Application
- Antibody Production (AbP), Standard (STD)
- Characteristics
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- Recombinant human Bcl-2-like 8 (full length, N-term HIS tag, transcript variant 2) protein expressed in E. coli.
- Produced with end-sequenced ORF clone
- Purity
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
- Top Product
- Discover our top product BAD Protein
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BCL2-Associated Agonist of Cell Death (BAD) (AA 69-168) protein (GST tag)
BAD Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
BCL2-Associated Agonist of Cell Death (BAD) (AA 1-168) protein (GST tag)BAD Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
BCL2-Associated Agonist of Cell Death (BAD) (AA 1-168) protein (Strep Tag)Crystallography grade BAD Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
BCL2-Associated Agonist of Cell Death (BAD) (AA 1-204) protein (Strep Tag)Crystallography grade BAD Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
BCL2-Associated Agonist of Cell Death (BAD) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)BAD Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - Comment
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The tag is located at the N-terminal.
- Restrictions
- For Research Use only
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- Concentration
- 50 μg/mL
- Buffer
- 25 mM Tris, pH 8.0, 150 mM NaCl, 10 % glycerol, 1 % Sarkosyl. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Target
- BAD (BCL2-Associated Agonist of Cell Death (BAD))
- Alternative Name
- Bcl-2-Like 8 (BAD Products)
- Synonyms
- bad Protein, fa01b12 Protein, wu:fa01b12 Protein, wu:fa96d04 Protein, BAD Protein, BBC2 Protein, BCL2L8 Protein, AI325008 Protein, Bbc2 Protein, BCL2-associated agonist of cell death b Protein, BCL2 associated agonist of cell death Protein, BCL2-associated agonist of cell death Protein, badb Protein, BAD Protein, Bad Protein
- Background
- The protein encoded by this gene is a member of the BCL-2 family. BCL-2 family members are known to be regulators of programmed cell death. This protein positively regulates cell apoptosis by forming heterodimers with BCL-xL and BCL-2, and reversing their death repressor activity. Proapoptotic activity of this protein is regulated through its phosphorylation. Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin were found to be involved in the regulation of this protein. Alternative splicing of this gene results in two transcript variants which encode the same isoform.
- Molecular Weight
- 18.2 kDa
- NCBI Accession
- NP_116784
- Pathways
- MAPK Signaling, PI3K-Akt Signaling, RTK Signaling, Apoptosis, Fc-epsilon Receptor Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Hepatitis C, CXCR4-mediated Signaling Events
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