GDF6 Protein

Catalog No. ABIN2715291

Product Details

Target: GDF6 (Growth Differentiation Factor 6 (GDF6))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Application: Functional Studies (Func), Antibody Production (AbP), Protein Interaction (PI), Standard (STD)

Specificity: Optimal preservation of protein structure, post-translational modifications and functions.

Characteristics:

• Recombinant human BMP13 / GDF6 protein expressed in E. coli.
• Produced with end-sequenced ORF clone
• Tested for bioactivity.

Purity: > 95 % as determined by SDS-PAGE and Coomassie blue staining

Endotoxin Level: Endotoxin level is <0.1 ng/μg of protein (<1EU/μg).

Biological Activity Comment: ED50 was determined by its ability to induce alkaline phosphatase production by ATDC-5 chondrogenic cells in the range of 2.0-3.0 ug/ml.

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays

Restrictions: For Research Use only

Handling

Buffer: Lyophilized from a 0.2 μM filtered solution of 20 mM phosphate buffer,100 mM NaCl, pH 7.2

Handling Advice: Resuspend the protein in the desired concentration in proper buffer

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: GDF6 (Growth Differentiation Factor 6 (GDF6))

Alternative Name: Bmp13,gdf6 (GDF6 Products)

Synonyms: BMP13 Protein, CDMP2 Protein, KFM Protein, KFS Protein, KFS1 Protein, KFSL Protein, LCA17 Protein, MCOP4 Protein, MCOPCB6 Protein, SCDO4 Protein, SGM1 Protein, GDF16 Protein, gdf16 Protein, CDMP-2 Protein, gdf6 Protein, rdr Protein, sb:cb278 Protein, bmp13 Protein, cdmp2 Protein, gdf-6 Protein, kfs Protein, kfsl Protein, sgm1 Protein, growth differentiation factor 6 Protein, growth differentiation factor 6a Protein, growth differentiation factor 6 S homeolog Protein, GDF6 Protein, Gdf6 Protein, gdf6 Protein, gdf6a Protein, gdf6.S Protein

Background: This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. This protein is required for normal formation of some bones and joints in the limbs, skull, and axial skeleton. Mutations in this gene are associated with Klippel-Feil syndrome, microphthalmia, and Leber congenital amaurosis.

Molecular Weight: 27 kDa

NCBI Accession: NP_001001557