DICER1 Protein (Transcript Variant 1) (Myc-DYKDDDDK Tag)

Catalog No. ABIN2719477

Product Details

Target: DICER1 (Dicer 1, Ribonuclease Type III (DICER1))

Protein Type: Recombinant

Protein Characteristics: Transcript Variant 1

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This DICER1 protein is labelled with Myc-DYKDDDDK Tag.

Application: Antibody Production (AbP), Standard (STD)

Characteristics:

• Recombinant human DICER1 (transcript variant 1) protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

References

Zhang, Li, Deng, Liu, Chen, Tang, Wang, Cao, Fang, Wen, Xu, Chen, Shi, Li, Xie, Tang: "Dicer interacts with SIRT7 and regulates H3K18 deacetylation in response to DNA damaging agents." in: Nucleic acids research, Vol. 44, Issue 8, pp. 3629-42, (2016) (PubMed).

Target Details

Target: DICER1 (Dicer 1, Ribonuclease Type III (DICER1))

Alternative Name: Dicer1 (DICER1 Products)

Synonyms: dcr1 Protein, dicer Protein, herna Protein, DICER1 Protein, dicer1 Protein, 1110006F08Rik Protein, D12Ertd7e Protein, mKIAA0928 Protein, DCR1 Protein, Dicer Protein, HERNA Protein, MNG1 Protein, DICER Protein, fc39d11 Protein, fc74b05 Protein, wu:fc39d11 Protein, wu:fc74b05 Protein, dicer 1, ribonuclease III Protein, endoribonuclease Dicer Protein, dicer 1, ribonuclease III S homeolog Protein, dicer 1, ribonuclease type III Protein, dicer 1 ribonuclease III Protein, DICER1 Protein, CpipJ_CPIJ010534 Protein, dicer1 Protein, dicer1.S Protein, Dicer1 Protein

Background: This gene encodes a protein possessing an RNA helicase motif containing a DEXH box in its amino terminus and an RNA motif in the carboxy terminus. The encoded protein functions as a ribonuclease and is required by the RNA interference and small temporal RNA (stRNA) pathways to produce the active small RNA component that represses gene expression. Alternative splicing results in multiple transcript variants.

Molecular Weight: 218.5 kDa

NCBI Accession: NP_803187

Pathways: Regulatory RNA Pathways, Stem Cell Maintenance, Ribonucleoprotein Complex Subunit Organization