LIG3 Protein (Myc-DYKDDDDK Tag)

Catalog No. ABIN2719546

Product Details

Target: LIG3 (Ligase III, DNA, ATP-Dependent (LIG3))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This LIG3 protein is labelled with Myc-DYKDDDDK Tag.

Application: Antibody Production (AbP), Standard (STD)

Characteristics:

• Recombinant human DNA ligase 3 (transcript variant alpha) protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: LIG3 (Ligase III, DNA, ATP-Dependent (LIG3))

Alternative Name: Dna Ligase 3 (LIG3 Products)

Synonyms: orf36 Protein, DDBDRAFT_0218523 Protein, DDBDRAFT_0232266 Protein, DDB_0218523 Protein, DDB_0232266 Protein, LIG3 Protein, wu:fb12c02 Protein, wu:fd10b03 Protein, wu:fm79b09 Protein, LIG2 Protein, D11Wsu78e Protein, An02g06890 Protein, AO090003000668 Protein, lig3 Protein, DNA ligase III Protein, BRCT domain-containing protein Protein, DNA ligase 3 Protein, ligase III, DNA, ATP-dependent Protein, ligase III, DNA, ATP-dependent L homeolog Protein, orf36 Protein, lig3 Protein, LIG3 Protein, Lig3 Protein, Bm1_22840 Protein, ANI_1_2644024 Protein, AOR_1_1174154 Protein, LOC100634887 Protein, lig3.L Protein

Background: This gene is a member of the DNA ligase family. Each member of this family encodes a protein that catalyzes the joining of DNA ends but they each have a distinct role in DNA metabolism. The protein encoded by this gene is involved in excision repair and is located in both the mitochondria and nucleus, with translation initiation from the upstream start codon allowing for transport to the mitochondria and translation initiation from a downstream start codon allowing for transport to the nucleus. Additionally, alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Molecular Weight: 108.6 kDa

NCBI Accession: NP_039269

Pathways: DNA Damage Repair