DOHH Protein (Transcript Variant 1) (Myc-DYKDDDDK Tag)
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- Target See all DOHH Proteins
- DOHH (Deoxyhypusine Hydroxylase/monooxygenase (DOHH))
- Protein Type
- Recombinant
- Protein Characteristics
- Transcript Variant 1
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This DOHH protein is labelled with Myc-DYKDDDDK Tag.
- Application
- Antibody Production (AbP), Standard (STD)
- Characteristics
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- Recombinant human DOHH / HLRC1 (transcript variant 1) protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
- Purity
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
- Top Product
- Discover our top product DOHH Protein
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Deoxyhypusine Hydroxylase/monooxygenase (DOHH) (AA 1-302) protein (GST tag)
DOHH Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Deoxyhypusine Hydroxylase/monooxygenase (DOHH) (AA 1-302) protein (Strep Tag)Crystallography grade DOHH Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
Deoxyhypusine Hydroxylase/monooxygenase (DOHH) (AA 1-302) protein (Strep Tag)Crystallography grade DOHH Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
Deoxyhypusine Hydroxylase/monooxygenase (DOHH) (Transcript Variant 2) protein (Myc-DYKDDDDK Tag)DOHH Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - Comment
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The tag is located at the C-terminal.
- Restrictions
- For Research Use only
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- Concentration
- 50 μg/mL
- Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Target
- DOHH (Deoxyhypusine Hydroxylase/monooxygenase (DOHH))
- Alternative Name
- Dohh,hlrc1 (DOHH Products)
- Synonyms
- HLRC1 Protein, hDOHH Protein, 1110033C18Rik Protein, AI585913 Protein, AL022869 Protein, Hlrc1 Protein, RGD1304783 Protein, fb33c04 Protein, hlrc1 Protein, wu:fb33c04 Protein, zgc:56338 Protein, deoxyhypusine hydroxylase Protein, deoxyhypusine hydroxylase/monooxygenase Protein, DOHH Protein, Dohh Protein, dohh Protein
- Background
- This gene encodes a metalloenzyme that catalyzes the last step in the conversion of lysine to the unique amino acid hypusine in eukaryotic initiation factor 5A. The encoded protein hydroxylates deoxyhypusine to form hypusine in the mature eukaryotic initiation factor 5A protein. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Feb 2009]
- Molecular Weight
- 32.7 kDa
- NCBI Accession
- NP_001138637
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