ECH1 Protein (Myc-DYKDDDDK Tag)

Catalog No. ABIN2719918

Product Details

Target: ECH1 (Enoyl Coenzyme A Hydratase 1, Peroxisomal (ECH1))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This ECH1 protein is labelled with Myc-DYKDDDDK Tag.

Application: Antibody Production (AbP), Standard (STD)

Characteristics:

• Recombinant human ECH1 protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: ECH1 (Enoyl Coenzyme A Hydratase 1, Peroxisomal (ECH1))

Alternative Name: Ech1 (ECH1 Products)

Synonyms: HPXEL Protein, Pxel Protein, AA617331 Protein, enoyl-CoA hydratase 1, peroxisomal Protein, enoyl CoA hydratase 1, peroxisomal Protein, enoyl-CoA hydratase 1 Protein, delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial Protein, enoyl-CoA hydratase 1, peroxisomal L homeolog Protein, enoyl coenzyme A hydratase 1, peroxisomal Protein, ech1 Protein, ECH1 Protein, LOC747511 Protein, ech1.L Protein, Ech1 Protein

Background: This gene encodes a member of the hydratase/isomerase superfamily. The gene product shows high sequence similarity to enoyl-coenzyme A (CoA) hydratases of several species, particularly within a conserved domain characteristic of these proteins. The encoded protein, which contains a C-terminal peroxisomal targeting sequence, localizes to the peroxisome. The rat ortholog, which localizes to the matrix of both the peroxisome and mitochondria, can isomerize 3-trans,5-cis-dienoyl-CoA to 2-trans,4-trans-dienoyl-CoA, indicating that it is a delta3,5-delta2,4-dienoyl-CoA isomerase. This enzyme functions in the auxiliary step of the fatty acid beta-oxidation pathway. Expression of the rat gene is induced by peroxisome proliferators.

Molecular Weight: 35.6 kDa

NCBI Accession: NP_001389

Pathways: Monocarboxylic Acid Catabolic Process