PHD1 Protein (Transcript Variant 1) (Myc-DYKDDDDK Tag)

Catalog No. ABIN2720027

Product Details

Target: PHD1 (EGLN2) (Egl-9 Family Hypoxia Inducible Factor 2 (EGLN2))

Protein Type: Recombinant

Protein Characteristics: Transcript Variant 1

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This PHD1 protein is labelled with Myc-DYKDDDDK Tag.

Application: Antibody Production (AbP), Standard (STD)

Characteristics:

• Recombinant human EGLN2 / PHD1 (transcript variant 1) protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

References

Lin, Li, Xing, Hu, Liang, Han, Wang, Hawke, Wang, Zhang, Wei, Ma, Park, Zhou, Zhou, Hu, Zhou, Marks, Liang, Hung, Lin, Yang: "The LINK-A lncRNA activates normoxic HIF1α signalling in triple-negative breast cancer." in: Nature cell biology, Vol. 18, Issue 2, pp. 213-24, (2016) (PubMed).

Target Details

Target: PHD1 (EGLN2) (Egl-9 Family Hypoxia Inducible Factor 2 (EGLN2))

Alternative Name: Egln2,phd1 (EGLN2 Products)

Synonyms: egln2 Protein, phd2 Protein, PHD1 Protein, 0610011A13Rik Protein, C85656 Protein, Hif-p4h-1 Protein, Ier4 Protein, Phd1 Protein, SM-20 Protein, HIF-PH1 Protein, HPH-1 Protein, HPH-3 Protein, PHD-1 Protein, EIT6 Protein, HIFPH1 Protein, egl-9 family hypoxia-inducible factor 1 Protein, egl-9 family hypoxia inducible factor 2 Protein, Egl nine homolog 2 Protein, egl-9 family hypoxia-inducible factor 2 L homeolog Protein, egl-9 family hypoxia-inducible factor 2 Protein, egln1 Protein, EGLN2 Protein, egln2 Protein, egln2.L Protein, Egln2 Protein

Background: The hypoxia inducible factor (HIF) is a transcriptional complex that is involved in oxygen homeostasis. At normal oxygen levels, the alpha subunit of HIF is targeted for degration by prolyl hydroxylation. This gene encodes an enzyme responsible for this post-translational modification. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the upstream RAB4B (RAB4B, member RAS oncogene family) gene.

Molecular Weight: 43.5 kDa

NCBI Accession: NP_444274

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Cell RedoxHomeostasis