FEN1 Protein (Myc-DYKDDDDK Tag)
-
- Target See all FEN1 Proteins
- FEN1 (Flap Structure-Specific Endonuclease 1 (FEN1))
- Protein Type
- Recombinant
-
Origin
- Human
-
Source
- HEK-293 Cells
- Purification tag / Conjugate
- This FEN1 protein is labelled with Myc-DYKDDDDK Tag.
- Application
- Antibody Production (AbP), Standard (STD)
- Characteristics
-
- Recombinant human FEN1 / RAD2 protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
- Purity
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
- Top Product
- Discover our top product FEN1 Protein
-
Flap Structure-Specific Endonuclease 1 (FEN1) (AA 1-110) protein (GST tag)
FEN1 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
Flap Structure-Specific Endonuclease 1 (FEN1) (AA 1-380) protein (GST tag)FEN1 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
Flap Structure-Specific Endonuclease 1 (FEN1) (AA 1-380) protein (His tag)Crystallography grade FEN1 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, SDS, WB, Crys
Flap Structure-Specific Endonuclease 1 (FEN1) (AA 1-378) protein (His tag)Crystallography grade FEN1 Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, SDS, WB, Crys
-
- Application Notes
-
Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - Comment
-
The tag is located at the C-terminal.
- Restrictions
- For Research Use only
-
- Concentration
- 50 μg/mL
- Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
-
- Target
- FEN1 (Flap Structure-Specific Endonuclease 1 (FEN1))
- Alternative Name
- Fen1,rad2 (FEN1 Products)
- Synonyms
- FEN-1 Protein, FEN1 Protein, AW538437 Protein, MF1 Protein, RAD2 Protein, cb879 Protein, fen-1 Protein, fen1 Protein, mf1 Protein, rad2 Protein, xFEN1a Protein, flap structure-specific endonuclease 1 Protein, flap endonuclease 1-like Protein, Flap endonuclease-1 Protein, 5'-3' exonuclease family protein Protein, flap structure specific endonuclease 1 Protein, flap structure-specific endonuclease 1 L homeolog Protein, nuclease, Rad2 family Protein, FEN1 Protein, LOC100808044 Protein, Bm1_49605 Protein, AT5G26680 Protein, Fen1 Protein, fen1 Protein, fen1.L Protein
- Background
- The protein encoded by this gene removes 5&apos overhanging flaps in DNA repair and processes the 5&apos ends of Okazaki fragments in lagging strand DNA synthesis. Direct physical interaction between this protein and AP endonuclease 1 during long-patch base excision repair provides coordinated loading of the proteins onto the substrate, thus passing the substrate from one enzyme to another. The protein is a member of the XPG/RAD2 endonuclease family and is one of ten proteins essential for cell-free DNA replication. DNA secondary structure can inhibit flap processing at certain trinucleotide repeats in a length-dependent manner by concealing the 5&apos end of the flap that is necessary for both binding and cleavage by the protein encoded by this gene. Therefore, secondary structure can deter the protective function of this protein, leading to site-specific trinucleotide expansions.
- Molecular Weight
- 42.4 kDa
- NCBI Accession
- NP_004102
- Pathways
- Telomere Maintenance, DNA Damage Repair, DNA Replication, Synthesis of DNA
-
