FGF20 Protein
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- Target See all FGF20 Proteins
- FGF20 (Fibroblast Growth Factor 20 (FGF20))
- Protein Type
- Recombinant
- Biological Activity
- Active
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Application
- Functional Studies (Func), Standard (STD), Antibody Production (AbP), Protein Interaction (PI)
- Specificity
- Optimal preservation of protein structure, post-translational modifications and functions.
- Characteristics
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- Recombinant human FGF20 protein expressed in E. coli.
- Produced with end-sequenced ORF clone
- Tested for bioactivity.
- Purity
- > 90 % as determined by SDS-PAGE and Coomassie blue staining
- Endotoxin Level
- Endotoxin level is <0.1 ng/μg of protein (<1EU/μg).
- Biological Activity Comment
- ED50 as determined by the dose-dependent stimulation of thymidine uptake by BaF3 cells expressing FGF-receptors is less than or equal to 10 ng/ml, corresponding to a specific activity of > 1 x 10^5 units/mg.
- Top Product
- Discover our top product FGF20 Protein
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Fibroblast Growth Factor 20 (FGF20) (Active) protein
FGF20 Origin: Human Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS-PAGE Func Active
Fibroblast Growth Factor 20 (FGF20) (AA 3-211) protein (His tag)FGF20 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Fibroblast Growth Factor 20 (FGF20) (AA 1-211) protein (Strep Tag)Crystallography grade FGF20 Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays - Restrictions
- For Research Use only
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- Buffer
- Lyophilized from a 0.2 μM filtered solution of 20 mM phosphate buffer,100 mM NaCl, pH 7.2
- Handling Advice
- Resuspend the protein in the desired concentration in proper buffer
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Target
- FGF20 (Fibroblast Growth Factor 20 (FGF20))
- Alternative Name
- Fgf20 (FGF20 Products)
- Synonyms
- FGF-20 Protein, fgf20 Protein, fgf20.S Protein, xfgf-20 Protein, xfgf20 Protein, dob Protein, wu:fb05c08 Protein, fibroblast growth factor 20 Protein, fibroblast growth factor 20 L homeolog Protein, fibroblast growth factor 20a Protein, FGF20 Protein, Fgf20 Protein, fgf20.L Protein, fgf20a Protein
- Background
- The protein encoded by this gene is a member of the fibroblast growth factor family. The fibroblast growth factors possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This gene product is a secreted neurotrophic factor but lacks a typical signal peptide. It is expressed in normal brain, particularly the cerebellum, and may regulate central nervous system development and function. Homodimerization of this protein was shown to regulate its receptor binding activity and concentration gradient in the extracellular matrix. Genetic variations of this gene have been associated with Parkinson disease susceptibility.
- Molecular Weight
- 23.2 kDa
- NCBI Accession
- NP_062825
- Pathways
- Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
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