FUS Protein (Myc-DYKDDDDK Tag)

Catalog No. ABIN2721461

Product Details

Target: FUS (Fused in Sarcoma (FUS))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This FUS protein is labelled with Myc-DYKDDDDK Tag.

Application: Antibody Production (AbP), Standard (STD)

Characteristics:

• Recombinant human Fusion (involved in t(12,16) in malignant liposarcoma) (FUS) protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

References

Bogaert, Boeynaems, Kato, Guo, Caulfield, Steyaert, Scheveneels, Wilmans, Haeck, Hersmus, Schymkowitz, Rousseau, Shorter, Callaerts, Robberecht, Van Damme, Van Den Bosch: "Molecular Dissection of FUS Points at Synergistic Effect of Low-Complexity Domains in Toxicity." in: Cell reports, Vol. 24, Issue 3, pp. 529-537.e4, (2018) (PubMed).

Gorthi, Romero, Loranc, Cao, Lawrence, Goodale, Iniguez, Bernard, Masamsetti, Roston, Lawlor, Toretsky, Stegmaier, Lessnick, Chen, Bishop: "EWS-FLI1 increases transcription to cause R-loops and block BRCA1 repair in Ewing sarcoma." in: Nature, Vol. 555, Issue 7696, pp. 387-391, (2018) (PubMed).

Blokhuis, Koppers, Groen, van den Heuvel, Dini Modigliani, Anink, Fumoto, van Diggelen, Snelting, Sodaar, Verheijen, Demmers, Veldink, Aronica, Bozzoni, den Hertog, van den Berg, Pasterkamp: "Comparative interactomics analysis of different ALS-associated proteins identifies converging molecular pathways." in: Acta neuropathologica, Vol. 132, Issue 2, pp. 175-96, (2016) (PubMed).

Altmeyer, Neelsen, Teloni, Pozdnyakova, Pellegrino, Grøfte, Rask, Streicher, Jungmichel, Nielsen, Lukas: "Liquid demixing of intrinsically disordered proteins is seeded by poly(ADP-ribose)." in: Nature communications, Vol. 6, pp. 8088, (2015) (PubMed).

Groen, Fumoto, Blokhuis, Engelen-Lee, Zhou, van den Heuvel, Koppers, van Diggelen, van Heest, Demmers, Kirby, Shaw, Aronica, Spliet, Veldink, van den Berg, Pasterkamp: "ALS-associated mutations in FUS disrupt the axonal distribution and function of SMN." in: Human molecular genetics, Vol. 22, Issue 18, pp. 3690-704, (2013) (PubMed).

Target Details

Target: FUS (Fused in Sarcoma (FUS))

Alternative Name: Fusion (Involved in T(12,16) in Malignant Liposarcoma) (Fus) (FUS Products)

Target Type: Viral Protein

Background: This gene encodes a multifunctional protein component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complex. The hnRNP complex is involved in pre-mRNA splicing and the export of fully processed mRNA to the cytoplasm. This protein belongs to the FET family of RNA-binding proteins which have been implicated in cellular processes that include regulation of gene expression, maintenance of genomic integrity and mRNA/microRNA processing. Alternative splicing results in multiple transcript variants. Defects in this gene result in amyotrophic lateral sclerosis type 6.

Molecular Weight: 53.2 kDa

NCBI Accession: NP_004951