GCLM Protein (Myc-DYKDDDDK Tag)
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- Target See all GCLM Proteins
- GCLM (Glutamate-Cysteine Ligase, Modifier Subunit (GCLM))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This GCLM protein is labelled with Myc-DYKDDDDK Tag.
- Application
- Antibody Production (AbP), Standard (STD)
- Characteristics
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- Recombinant human GCLM / GLCLR protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
- Purity
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
- Top Product
- Discover our top product GCLM Protein
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Glutamate-Cysteine Ligase, Modifier Subunit (GCLM) (AA 1-274) protein (GST tag)
GCLM Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Glutamate-Cysteine Ligase, Modifier Subunit (GCLM) (AA 1-274) protein (Strep Tag)Crystallography grade GCLM Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA
Glutamate-Cysteine Ligase, Modifier Subunit (GCLM) (AA 1-274) protein (Strep Tag)Crystallography grade GCLM Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - Comment
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The tag is located at the C-terminal.
- Restrictions
- For Research Use only
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- Concentration
- 50 μg/mL
- Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Target
- GCLM (Glutamate-Cysteine Ligase, Modifier Subunit (GCLM))
- Alternative Name
- Gclm,glclr (GCLM Products)
- Synonyms
- Glclr Protein, GLCLR Protein, id:ibd3182 Protein, wu:fi24c07 Protein, zgc:55903 Protein, AI649393 Protein, Gcmc Protein, glutamate-cysteine ligase regulatory subunit Protein, glutamate cysteine ligase, modifier subunit Protein, glutamate-cysteine ligase modifier subunit Protein, glutamate-cysteine ligase, modifier subunit Protein, glutamate-cysteine ligase, modifier subunit L homeolog Protein, PTRG_09814 Protein, Gclm Protein, GCLM Protein, gclm Protein, gclm.L Protein
- Background
- Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase, is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. Gamma glutamylcysteine synthetase deficiency has been implicated in some forms of hemolytic anemia. Alternative splicing results in multiple transcript variants encoding different isoforms.
- Molecular Weight
- 30.5 kDa
- NCBI Accession
- NP_002052
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