IFIH1 Protein (Myc-DYKDDDDK Tag)

Catalog No. ABIN2722584

Product Details

Target: IFIH1 (Interferon Induced with Helicase C Domain 1 (IFIH1))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This IFIH1 protein is labelled with Myc-DYKDDDDK Tag.

Application: Antibody Production (AbP), Standard (STD)

Characteristics:

• Recombinant human Helicard protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

References

Miro, Laaref, Rofidal, Lagrafeuille, Hem, Thorel, Méchin, Mamchaoui, Mouly, Claustres, Tuffery-Giraud: "FUBP1: a new protagonist in splicing regulation of the DMD gene." in: Nucleic acids research, Vol. 43, Issue 4, pp. 2378-89, (2015) (PubMed).

Hua, Yuan, Li, Coursey, Pflugfelder, Li: "A Novel Innate Response of Human Corneal Epithelium to Heat-killed Candida albicans by Producing Peptidoglycan Recognition Proteins." in: PLoS ONE, Vol. 10, Issue 6, pp. e0128039, (2015) (PubMed).

Choi, Lakamp-Hawley, Kolar, Yan, Borgstahl, Ouellette: "The OB-fold domain 1 of human POT1 recognizes both telomeric and non-telomeric DNA motifs." in: Biochimie, Vol. 115, pp. 17-27, (2015) (PubMed).

Mellett, Atzei, Bergin, Horgan, Floss, Wurst, Callanan, Moynagh: "Orphan receptor IL-17RD regulates Toll-like receptor signalling via SEFIR/TIR interactions." in: Nature communications, Vol. 6, pp. 6669, (2015) (PubMed).

Labrador-Horrillo, Martinez, Selva-OCallaghan, Trallero-Araguas, Balada, Vilardell-Tarres, Juárez: "Anti-MDA5 antibodies in a large Mediterranean population of adults with dermatomyositis." in: Journal of immunology research, Vol. 2014, pp. 290797, (2014) (PubMed).

Tansley, Betteridge, Gunawardena, Jacques, Owens, Pilkington, Arnold, Yasin, Moraitis, Wedderburn, McHugh: "Anti-MDA5 autoantibodies in juvenile dermatomyositis identify a distinct clinical phenotype: a prospective cohort study." in: Arthritis research & therapy, Vol. 16, Issue 4, pp. R138, (2014) (PubMed).

Ma, Paik, Barile: "Bioactive lysophospholipids generated by hepatic lipase degradation of lipoproteins lead to complement activation via the classical pathway." in: Investigative ophthalmology & visual science, Vol. 55, Issue 10, pp. 6187-93, (2014) (PubMed).

Fiandaca, Kapogiannis, Mapstone, Boxer, Eitan, Schwartz, Abner, Petersen, Federoff, Miller, Goetzl: "Identification of preclinical Alzheimer's disease by a profile of pathogenic proteins in neurally derived blood exosomes: A case-control study." in: Alzheimer's & dementia : the journal of the Alzheimer's Association, (2014) (PubMed).

Target Details

Target: IFIH1 (Interferon Induced with Helicase C Domain 1 (IFIH1))

Alternative Name: Helicard (IFIH1 Products)

Synonyms: MDA5 Protein, Hlcd Protein, IDDM19 Protein, MDA-5 Protein, RLR-2 Protein, 9130009C22Rik Protein, Helicard Protein, RGD1560496 Protein, interferon induced with helicase C domain 1 Protein, IFIH1 Protein, Ifih1 Protein

Background: DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein that is upregulated in response to treatment with beta-interferon and a protein kinase C-activating compound, mezerein. Irreversible reprogramming of melanomas can be achieved by treatment with both these agents treatment with either agent alone only achieves reversible differentiation. Genetic variation in this gene is associated with diabetes mellitus insulin-dependent type 19.

Molecular Weight: 116.5 kDa

NCBI Accession: NP_071451

Pathways: Activation of Innate immune Response