IFNB1 Protein

Catalog No. ABIN2723332

Product Details

Target: IFNB1 (Interferon, beta 1, Fibroblast (IFNB1))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: CHO Cells

Application: Functional Studies (Func), Antibody Production (AbP), Protein Interaction (PI), Standard (STD)

Specificity: Optimal preservation of protein structure, post-translational modifications and functions.

Characteristics:

• Recombinant human IFNB / Interferon beta protein expressed in CHO cells.
• Produced with end-sequenced ORF clone
• Tested for bioactivity.

Purity: > 95 % as determined by SDS-PAGE and Coomassie blue staining

Endotoxin Level: Endotoxin level is <0.1 ng/μg of protein (<1EU/μg).

Biological Activity Comment: Assay #1:Measured by its ability to induce apoptosis in HeLa cells. The expected ED50 for this effect is 20-30 ng/ml. Assay #2:Determined by its ability to stimulate the proliferation of human TF-1 cells. The expected ED50 is less than or equal to 0.1 ng/ml, corresponding to a specific activity of > 1 x 10^7 units/mg

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays

Restrictions: For Research Use only

Handling

Buffer: Lyophilized from a 0.2 μM filtered solution of 20 mM phosphate buffer,100 mM NaCl, pH 7.2

Handling Advice: Centrifuge the vial prior to opening. Reconstitute the protein in water to a concnetration of 0.1 ~ 1.0 mg/mL. Do not vertex. For extended storage, it is recommended to further dilute in a proper buffer (i.e. water or PBS) containing a carrier protein such as 0.1 % BSA, and store in working aliquots at -20 °C or -80 °C

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: IFNB1 (Interferon, beta 1, Fibroblast (IFNB1))

Alternative Name: Ifnb,interferon beta (IFNB1 Products)

Synonyms: IFB Protein, IFF Protein, IFNB Protein, IFN-beta Protein, Ifb Protein, Ifnb Protein, IFNb Protein, interferon beta 1 Protein, interferon beta 1, fibroblast Protein, interferon, beta 1, fibroblast Protein, IFNB1 Protein, Ifnb1 Protein

Background: Has antiviral, antibacterial and anticancer activities. [UniProtKB/Swiss-Prot Function]

Molecular Weight: 20 kDa

NCBI Accession: NP_002167

Pathways: JAK-STAT Signaling, TCR Signaling, TLR Signaling, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Autophagy, Inflammasome