Inter-alpha Globulin Inhibitor H2 Polypeptide (ITIH2) protein (Myc-DYKDDDDK Tag) Protein
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- Target
- ITIH2
- Protein Type
- Recombinant
- Origin
- Human
- Source
- HEK-293 Cells
- Purification tag / Conjugate
- This ITIH2 protein is labelled with Myc-DYKDDDDK Tag.
- Application
- Antibody Production (AbP), Functional Studies (Func), Protein Interaction (PI), Standard (STD)
- Specificity
- Optimal preservation of protein structure, post-translational modifications and functions.
- Characteristics
- Recombinant human ITIH2 protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
- Purity
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
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Inter-alpha Globulin Inhibitor H2 Polypeptide (ITIH2) (AA 55-702) protein (His tag) Protein
ITIH2 Origin: Human Source: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. Crys, ELISA, SDS, WB
Inter-alpha Globulin Inhibitor H2 Polypeptide (ITIH2) (AA 55-702) protein (His tag) ProteinITIH2 Origin: Mouse Source: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. Crys, ELISA, SDS, WB
Inter-alpha Globulin Inhibitor H2 Polypeptide (ITIH2) (AA 452-702) protein (His tag,T7 tag) ProteinITIH2 Origin: Human Source: Escherichia coli (E. coli) Recombinant > 95 % IP, SDS, ELISA, WB
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- Application Notes
- Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays - Comment
The tag is located at the C-terminal.
- Restrictions
- For Research Use only
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- Concentration
- > 50 μg/mL
- Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Target
- ITIH2
- Synonyms
- AI747202, Intin2, Itih-2, H2P, SHAP, ITI-HC2, inter-alpha trypsin inhibitor, heavy chain 2, inter-alpha-trypsin inhibitor heavy chain 2, Itih2, ITIH2
- Background
- The inter-alpha-trypsin inhibitors (ITI) are a family of structurally related plasma serine protease inhibitors involved in extracellular matrix stabilization and in prevention of tumor metastasis. The ITI family contains multiple proteins made up of a light chain (see MIM 176870) and a variable number of heavy chains (Salier et al., 1987 [PubMed 2446322] Himmelfarb et al., 2004 [PubMed 14744536]).[supplied by OMIM, Nov 2009].
- Molecular Weight
- 106.3 kDa
- NCBI Accession
- NP_002207
- Pathways
- Glycosaminoglycan Metabolic Process