MED20 Protein (Myc-DYKDDDDK Tag)
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- Target See all MED20 Proteins
- MED20 (Mediator Complex Subunit 20 (MED20))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This MED20 protein is labelled with Myc-DYKDDDDK Tag.
- Application
- Antibody Production (AbP), Standard (STD)
- Characteristics
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- Recombinant human MED20 protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
- Purity
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
- Top Product
- Discover our top product MED20 Protein
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Mediator Complex Subunit 20 (MED20) (AA 1-212) protein (His tag)
MED20 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Mediator Complex Subunit 20 (MED20) (AA 1-212) protein (GST tag)MED20 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Mediator Complex Subunit 20 (MED20) (AA 1-212) protein (Strep Tag)Crystallography grade MED20 Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
Mediator Complex Subunit 20 (MED20) (AA 1-212) protein (Strep Tag)Crystallography grade MED20 Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - Comment
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The tag is located at the C-terminal.
- Restrictions
- For Research Use only
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- Concentration
- 50 μg/mL
- Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Target
- MED20 (Mediator Complex Subunit 20 (MED20))
- Alternative Name
- Med20 (MED20 Products)
- Synonyms
- PRO0213 Protein, TRFP Protein, 1110011O05Rik Protein, 2410115I17Rik Protein, AU018348 Protein, Trfp Protein, Usp49 Protein, trfp Protein, zgc:101043 Protein, mediator complex subunit 20 Protein, mediator complex subunit 20 S homeolog Protein, MED20 Protein, Med20 Protein, med20 Protein, med20.S Protein
- Background
- This gene encodes a component of the mediator complex (also known as TRAP, SMCC, DRIP, or ARC), a transcriptional coactivator complex thought to be required for the expression of almost all genes. The mediator complex is recruited by transcriptional activators or nuclear receptors to induce gene expression, by interacting with RNA polymerase II and promoting the formation of a transcriptional pre-initiation complex. A mutation in this gene has been associated with a novel infantile-onset neurodegenerative movement disorder. Alternatively spliced transcript variants have been identified.
- Molecular Weight
- 23 kDa
- NCBI Accession
- NP_004266
- Pathways
- Regulation of Lipid Metabolism by PPARalpha
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