MED25 Protein (Myc-DYKDDDDK Tag)
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- Target See all MED25 Proteins
- MED25 (Mediator Complex Subunit 25 (MED25))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This MED25 protein is labelled with Myc-DYKDDDDK Tag.
- Application
- Antibody Production (AbP), Standard (STD)
- Characteristics
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- Recombinant human MED25 protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
- Purity
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
- Top Product
- Discover our top product MED25 Protein
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Mediator Complex Subunit 25 (MED25) (AA 1-747) protein (Strep Tag)
Crystallography grade MED25 Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, SDS, WB
Mediator Complex Subunit 25 (MED25) (AA 1-745) protein (Strep Tag)Crystallography grade MED25 Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, SDS, WB
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - Comment
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The tag is located at the C-terminal.
- Restrictions
- For Research Use only
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- Concentration
- 50 μg/mL
- Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Target
- MED25 (Mediator Complex Subunit 25 (MED25))
- Abstract
- MED25 Products
- Synonyms
- Arc92 Protein, CG12254 Protein, Dmel\\CG12254 Protein, LD07688 Protein, Med25 Protein, dARC92 Protein, dDrip97 Protein, med25 Protein, ACID1 Protein, ARC92 Protein, CMT2B2 Protein, P78 Protein, PTOV2 Protein, RGD1305137 Protein, 2610034E13Rik Protein, 2610529E18Rik Protein, ESTM2 Protein, X83308 Protein, zgc:63555 Protein, Mediator complex subunit 25 Protein, mediator complex subunit 25 Protein, mediator complex subunit 25 S homeolog Protein, MED25 Protein, med25 Protein, Med25 Protein, med25.S Protein
- Background
- This gene encodes a component of the transcriptional coactivator complex termed the Mediator complex. This complex is required for transcription of most RNA polymerase II-dependent genes. The encoded protein plays a role in chromatin modification and in preinitiation complex assembly. Mutations in this gene are associated with Charcot-Marie-Tooth disease type 2B2.
- Molecular Weight
- 78 kDa
- NCBI Accession
- NP_112235
- Pathways
- Nuclear Hormone Receptor Binding, Chromatin Binding, Regulation of Lipid Metabolism by PPARalpha, Production of Molecular Mediator of Immune Response
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