MRPL21 Protein (His tag)

Catalog No. ABIN2726342

Product Details

Target: MRPL21 (Mitochondrial Ribosomal Protein L21 (MRPL21))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This MRPL21 protein is labelled with His tag.

Application: Antibody Production (AbP), Standard (STD)

Characteristics:

• Recombinant human MRPL21 (full length, N-term HIS tag, transcript variant 4) protein expressed in E. coli.
• Produced with end-sequenced ORF clone

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the N-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris, pH 8.0, 150 mM NaCl, 10 % glycerol, 1 % Sarkosyl.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: MRPL21 (Mitochondrial Ribosomal Protein L21 (MRPL21))

Alternative Name: Mrpl21 (MRPL21 Products)

Synonyms: CG9730 Protein, Dmel\\CG9730 Protein, MRP-L21 Protein, MGC131341 Protein, DDBDRAFT_0188115 Protein, DDBDRAFT_0267021 Protein, DDB_0188115 Protein, DDB_0267021 Protein, L21mt Protein, BC028768 Protein, mitochondrial ribosomal protein L21 Protein, mitochondrial ribosomal protein L21 L homeolog Protein, ribosomal protein L21, mitochondrial Protein, Mrpl21 Protein, mRpL21 Protein, MRPL21 Protein, mrpl21 Protein, mrpl21.L Protein, mrpL21 Protein

Background: Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75 % protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. Multiple transcript variants encoding different isoforms were identified through sequence analysis although some may be subject to nonsense-mediated decay (NMD).

Molecular Weight: 22.6 kDa

NCBI Accession: NP_852615