MRPL50 Protein (His tag)

Catalog No. ABIN2726361

Product Details

Target: MRPL50 (Mitochondrial Ribosomal Protein L50 (MRPL50))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This MRPL50 protein is labelled with His tag.

Application: Antibody Production (AbP), Standard (STD)

Characteristics:

• Recombinant human MRPL50 (full length, N-term HIS tag) protein expressed in E. coli.
• Produced with end-sequenced ORF clone

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the N-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris, pH 8.0, 150 mM NaCl, 10 % glycerol, 1 % Sarkosyl. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: MRPL50 (Mitochondrial Ribosomal Protein L50 (MRPL50))

Alternative Name: Mrpl50 (MRPL50 Products)

Synonyms: ANON-65Eb Protein, BcDNA:GM06185 Protein, BcDNA:RE06140 Protein, CG8612 Protein, Dmel\\CG8612 Protein, MRP-L50 Protein, anon-65Eb Protein, DKFZp468B0910 Protein, AA408971 Protein, AI317351 Protein, D4Wsu125e Protein, mitochondrial ribosomal protein L50 Protein, mitochondrial 54S ribosomal protein MRPL50 Protein, mRpL50 Protein, Mrpl50 Protein, MRPL50 Protein

Background: Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75 % protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a putative 39S subunit protein and belongs to the L47P ribosomal protein family. Pseudogenes corresponding to this gene are found on chromosomes 2p, 2q, 5p, and 10q.

Molecular Weight: 18.3 kDa

NCBI Accession: NP_061924