RPS14 Protein (His tag)

Catalog No. ABIN2726374

Product Details

Target: RPS14 (Ribosomal Protein S14 (RPS14))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This RPS14 protein is labelled with His tag.

Application: Antibody Production (AbP), Standard (STD)

Characteristics:

• Recombinant human MRPS14 (full length, N-term HIS tag, transcript variant 1) protein expressed in E. coli.
• Produced with end-sequenced ORF clone

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the N-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris, pH 8.0, 150 mM NaCl, 10 % glycerol, 1 % Sarkosyl. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: RPS14 (Ribosomal Protein S14 (RPS14))

Alternative Name: Mrps14 (RPS14 Products)

Synonyms: EMTB Protein, S14 Protein, 2600014J02Rik Protein, AL023078 Protein, fa92e08 Protein, wu:fa92e08 Protein, zgc:73215 Protein, CG1527 Protein, Dmel\CG1527 Protein, RPS14B Protein, RpS14 Protein, RpS14B Protein, S14b Protein, anon-EST:Posey115 Protein, anon-EST:Posey131 Protein, anon-EST:Posey154 Protein, anon-EST:Posey77 Protein, rpS14B Protein, ribosomal protein S14 Protein, 40S ribosomal protein S14 Protein, ribosomal protein S14 L homeolog Protein, 30S ribosomal protein S14 Protein, Ribosomal protein S14b Protein, mitochondrial ribosomal protein S14 Protein, RPS14 Protein, Rps14 Protein, rps-14 Protein, rps14 Protein, rps14.L Protein, LOC100286294 Protein, RpS14 Protein, RpS14b Protein, MRPS14 Protein

Background: Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75 % protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that belongs to the ribosomal protein S14P family. Alternative splicing results in multiple transcript variants.

Molecular Weight: 15.1 kDa

NCBI Accession: NP_071383

Pathways: Ribonucleoprotein Complex Subunit Organization, Ribosome Assembly