MSTN Protein
-
- Target See all MSTN Proteins
- MSTN (Myostatin (MSTN))
- Protein Type
- Recombinant
- Biological Activity
- Active
-
Origin
- Human
-
Source
- Escherichia coli (E. coli)
- Application
- Functional Studies (Func), Standard (STD), Antibody Production (AbP), Protein Interaction (PI)
- Specificity
- Optimal preservation of protein structure, post-translational modifications and functions.
- Characteristics
-
- Recombinant human Myostatin protein expressed in E. coli.
- Produced with end-sequenced ORF clone
- Tested for bioactivity.
- Purity
- > 95 % as determined by SDS-PAGE and Coomassie blue staining
- Endotoxin Level
- Endotoxin level is <0.1 ng/μg of protein (<1EU/μg).
- Biological Activity Comment
- Determined by its ability to neutralize the Myostatin inhibitory effect of murine MPC-11 cells. The expected ED50 is 0.01-0.04 ug/mL in the presence of 50 ng/ml Myostatin.
- Top Product
- Discover our top product MSTN Protein
-
Myostatin (MSTN) (Active) protein
MSTN Origin: Human Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS-PAGE Func Active
Myostatin (MSTN) (AA 267-375) protein (His-IF2DI Tag)MSTN Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Myostatin (MSTN) (AA 1-375) protein (GST tag)MSTN Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Myostatin (MSTN) (AA 121-202) protein (GST tag)MSTN Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Myostatin (MSTN) (AA 241-310) protein (GST tag)MSTN Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Myostatin (MSTN) (AA 267-375) protein (His tag)Crystallography grade MSTN Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Myostatin (MSTN) (AA 268-376) protein (His tag)Crystallography grade MSTN Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
-
- Application Notes
-
Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays - Restrictions
- For Research Use only
-
- Buffer
- Lyophilized from a 0.2 μM filtered solution of 20 mM phosphate buffer,100 mM NaCl, pH 7.2
- Handling Advice
- Resuspend the protein in the desired concentration in proper buffer
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
-
- Target
- MSTN (Myostatin (MSTN))
- Alternative Name
- Myostatin (MSTN Products)
- Synonyms
- GDF-8 Protein, GDF8 Protein, gdf8 Protein, mstn Protein, zgc:109749 Protein, Gdf8 Protein, NV13143 Protein, MSLHP Protein, Cmpt Protein, myostatin Protein, myostatin b Protein, growth/differentiation factor 8 Protein, MSTN Protein, mstnb Protein, LOC5577632 Protein, BRAFLDRAFT_290840 Protein, Mstn Protein, mstn Protein
- Background
- This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. This protein negatively regulates skeletal muscle cell proliferation and differentiation. Mutations in this gene are associated with increased skeletal muscle mass in humans and other mammals.
- Molecular Weight
- 27.8 kDa
- NCBI Accession
- NP_005250
-
