NACA Protein (Transcript Variant 3) (Myc-DYKDDDDK Tag)
Quick Overview for NACA Protein (Transcript Variant 3) (Myc-DYKDDDDK Tag) (ABIN2726835)
Target
See all NACA (NACa1) ProteinsProtein Type
Origin
Source
Application
Purity
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Protein Characteristics
- Transcript Variant 3
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Purification tag / Conjugate
- This NACA protein is labelled with Myc-DYKDDDDK Tag.
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Characteristics
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- Recombinant human Nascent polypeptide-associated complex alpha subunit (NACA), transcript variant 3 (transcript variant 3) protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
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Nascent Polypeptide Associated Complex alpha 1 (NACa1) (AA 1-215) protein (His tag)
custom-made NACa1 Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Nascent Polypeptide Associated Complex alpha 1 (NACa1) (AA 1-215) protein (His tag)custom-made NACa1 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Nascent Polypeptide Associated Complex alpha 1 (NACa1) (AA 1-215) protein (His tag)NACa1 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Nascent Polypeptide Associated Complex alpha 1 (NACa1) (AA 1-215) protein (GST tag)NACa1 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Nascent Polypeptide Associated Complex alpha 1 (NACa1) (Transcript Variant 4) protein (Myc-DYKDDDDK Tag)NACa1 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
Nascent Polypeptide Associated Complex alpha 1 (NACa1) (Transcript Variant 2) protein (Myc-DYKDDDDK Tag)NACa1 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays -
Comment
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The tag is located at the C-terminal.
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Restrictions
- For Research Use only
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Concentration
- 50 μg/mL
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Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
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Storage
- -80 °C
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Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- NACA (NACa1) (Nascent Polypeptide Associated Complex alpha 1 (NACa1))
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Alternative Name
- Nascent Polypeptide-Associated Complex alpha Subunit (Naca)
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Background
- This gene encodes a protein that associates with basic transcription factor 3 (BTF3) to form the nascent polypeptide-associated complex (NAC). This complex binds to nascent proteins that lack a signal peptide motif as they emerge from the ribosome, blocking interaction with the signal recognition particle (SRP) and preventing mistranslocation to the endoplasmic reticulum. This protein is an IgE autoantigen in atopic dermatitis patients. Alternative splicing results in multiple transcript variants, but the full length nature of some of these variants, including those encoding very large proteins, has not been determined. There are multiple pseudogenes of this gene on different chromosomes.
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Molecular Weight
- 23.2 kDa
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NCBI Accession
- NP_005585
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Pathways
- Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development
Target
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