NOV Protein
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- Target See all NOV Proteins
- NOV (Nephroblastoma Overexpressed (NOV))
- Protein Type
- Recombinant
- Biological Activity
- Active
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Application
- Functional Studies (Func), Antibody Production (AbP), Protein Interaction (PI), Standard (STD)
- Specificity
- Optimal preservation of protein structure, post-translational modifications and functions.
- Characteristics
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- Recombinant human NOV protein expressed in E. coli.
- Produced with end-sequenced ORF clone
- Tested for bioactivity.
- Purity
- > 95 % as determined by SDS-PAGE and Coomassie blue staining
- Endotoxin Level
- Endotoxin level is <0.1 ng/μg of protein (<1EU/μg).
- Biological Activity Comment
- Determined by a cell proliferation assay using BALB/c 3T3 cells. The expected ED50 for this effect is 1.0-2.0 ug/mL
- Top Product
- Discover our top product NOV Protein
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Nephroblastoma Overexpressed (NOV) (AA 1-357) protein (GST tag)
NOV Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Nephroblastoma Overexpressed (NOV) protein (His tag)NOV Origin: Mouse Host: Human Cells Recombinant > 95 % as determined by SDS-PAGE
Nephroblastoma Overexpressed (NOV) (AA 28-357) protein (His tag)NOV Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Nephroblastoma Overexpressed (NOV) (AA 32-131) protein (GST tag)NOV Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Nephroblastoma Overexpressed (NOV) (AA 32-357) protein (His tag)Crystallography grade NOV Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Nephroblastoma Overexpressed (NOV) (AA 22-354) protein (His tag)Crystallography grade NOV Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays - Restrictions
- For Research Use only
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- Buffer
- Lyophilized from a 0.2 μM filtered solution of 20 mM phosphate buffer,100 mM NaCl, pH 7.2
- Handling Advice
- Resuspend the protein in the desired concentration in proper buffer
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Target
- NOV (Nephroblastoma Overexpressed (NOV))
- Alternative Name
- Nov (NOV Products)
- Synonyms
- CCN3 Protein, IBP-9 Protein, IGFBP-9 Protein, IGFBP9 Protein, NOVh Protein, nov-A Protein, xnov Protein, NovH Protein, C130088N23Rik Protein, nephroblastoma overexpressed Protein, nephroblastoma overexpressed L homeolog Protein, nephroblastoma overexpressed gene Protein, NOV Protein, nov.L Protein, Nov Protein
- Background
- The protein encoded by this gene is a small secreted cysteine-rich protein and a member of the CCN family of regulatory proteins. CNN family proteins associate with the extracellular matrix and play an important role in cardiovascular and skeletal development, fibrosis and cancer development.
- Molecular Weight
- 36.2 kDa
- NCBI Accession
- NP_002505
- Pathways
- Smooth Muscle Cell Migration, Growth Factor Binding
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