OCT4 Protein (Transcript Variant 1) (Myc-DYKDDDDK Tag)
(1 reference)-
- Target See all OCT4 (POU5F1) Proteins
- OCT4 (POU5F1) (POU Class 5 Homeobox 1 (POU5F1))
- Protein Type
- Recombinant
- Protein Characteristics
- Transcript Variant 1
-
Origin
- Human
-
Source
- HEK-293 Cells
- Purification tag / Conjugate
- This OCT4 protein is labelled with Myc-DYKDDDDK Tag.
- Application
- Antibody Production (AbP), Standard (STD)
- Characteristics
-
- Recombinant human OCT3/4 (transcript variant 1) protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
- Purity
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
- Top Product
- Discover our top product POU5F1 Protein
-
POU Class 5 Homeobox 1 (POU5F1) (AA 233-360) protein (His-IF2DI Tag)
POU5F1 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
POU Class 5 Homeobox 1 (POU5F1) (AA 1-360) protein (His tag)POU5F1 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
POU Class 5 Homeobox 1 (POU5F1) (AA 1-164) protein (GST tag)POU5F1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
POU Class 5 Homeobox 1 (POU5F1) (AA 1-360) protein (Strep Tag)Crystallography grade POU5F1 Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
POU Class 5 Homeobox 1 (POU5F1) (AA 1-352) protein (Strep Tag)Crystallography grade POU5F1 Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
-
- Application Notes
-
Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - Comment
-
The tag is located at the C-terminal.
- Restrictions
- For Research Use only
-
- Concentration
- 50 μg/mL
- Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
-
-
: "Pioneer transcription factors target partial DNA motifs on nucleosomes to initiate reprogramming." in: Cell, Vol. 161, Issue 3, pp. 555-68, (2015) (PubMed).
-
: "Pioneer transcription factors target partial DNA motifs on nucleosomes to initiate reprogramming." in: Cell, Vol. 161, Issue 3, pp. 555-68, (2015) (PubMed).
-
- Target
- OCT4 (POU5F1) (POU Class 5 Homeobox 1 (POU5F1))
- Alternative Name
- Oct3/4 (POU5F1 Products)
- Synonyms
- OCT3 Protein, OCT4 Protein, OTF-3 Protein, OTF3 Protein, OTF4 Protein, Oct-3 Protein, Oct-4 Protein, POU5F1 Protein, gp9 Protein, gp-9 Protein, oct4 Protein, pou2 Protein, POU-2 Protein, cb197 Protein, spg/pou2 Protein, chunp6868 Protein, wu:fd18d06 Protein, NF-A3 Protein, Oct-3/4 Protein, Oct3 Protein, Oct3/4 Protein, Oct4 Protein, Otf-3 Protein, Otf-4 Protein, Otf3 Protein, Otf3-rs7 Protein, Otf3g Protein, Otf4 Protein, POU class 5 homeobox 1 Protein, POU domain, class 5, transcription factor 3 Protein, POU domain, class 5, transcription factor 1 Protein, POU5F1 Protein, pou5f3 Protein, Pou5f1 Protein
- Background
- This gene encodes a transcription factor containing a POU homeodomain that plays a key role in embryonic development and stem cell pluripotency. Aberrant expression of this gene in adult tissues is associated with tumorigenesis. This gene can participate in a translocation with the Ewing&aposs sarcoma gene on chromosome 21, which also leads to tumor formation. Alternative splicing, as well as usage of alternative AUG and non-AUG translation initiation codons, results in multiple isoforms. One of the AUG start codons is polymorphic in human populations. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12.
- Molecular Weight
- 38.4 kDa
- NCBI Accession
- NP_002692
- Pathways
- Stem Cell Maintenance
-
