PCBP2 Protein (Transcript Variant 6) (Myc-DYKDDDDK Tag)

Catalog No. ABIN2728356

Product Details

Target: PCBP2 (Poly(rC) Binding Protein 2 (PCBP2))

Protein Type: Recombinant

Protein Characteristics: Transcript Variant 6

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This PCBP2 protein is labelled with Myc-DYKDDDDK Tag.

Application: Antibody Production (AbP), Standard (STD)

Characteristics:

• Recombinant human PCBP2 / hnRNP-E2 (transcript variant 6) protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: PCBP2 (Poly(rC) Binding Protein 2 (PCBP2))

Alternative Name: Pcbp2,hnrnp-e2 (PCBP2 Products)

Synonyms: HNRNPE2 Protein, HNRPE2 Protein, hnRNP-E2 Protein, AW412548 Protein, Hnrpx Protein, alphaCP-2 Protein, PCBP3 Protein, fb36h02 Protein, wu:fb36h02 Protein, zgc:55964 Protein, PCBP2 Protein, poly(rC) binding protein 2 Protein, poly(rC) binding protein 2 L homeolog Protein, PCBP2 Protein, Pcbp2 Protein, pcbp2 Protein, pcbp2.L Protein

Background: The protein encoded by this gene appears to be multifunctional. Along with PCBP-1 and hnRNPK, it is one of the major cellular poly(rC)-binding proteins. The encoded protein contains three K-homologous (KH) domains which may be involved in RNA binding. Together with PCBP-1, this protein also functions as a translational coactivator of poliovirus RNA via a sequence-specific interaction with stem-loop IV of the IRES, promoting poliovirus RNA replication by binding to its 5'-terminal cloverleaf structure. It has also been implicated in translational control of the 15-lipoxygenase mRNA, human papillomavirus type 16 L2 mRNA, and hepatitis A virus RNA. The encoded protein is also suggested to play a part in formation of a sequence-specific alpha-globin mRNP complex which is associated with alpha-globin mRNA stability. This multiexon structural mRNA is thought to be retrotransposed to generate PCBP-1, an intronless gene with functions similar to that of PCBP2. This gene and PCBP-1 have paralogous genes (PCBP3 and PCBP4) which are thought to have arisen as a result of duplication events of entire genes. Thsi gene also has two processed pseudogenes (PCBP2P1 and PCBP2P2). Multiple transcript variants encoding different isoforms have been found for this gene.

Molecular Weight: 35.2 kDa

NCBI Accession: NP_001122385