PFKFB3 Protein (His tag)
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- Target See all PFKFB3 Proteins
- PFKFB3 (6-phosphofructo-2-Kinase/fructose-2,6-Biphosphatase 3 (PFKFB3))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This PFKFB3 protein is labelled with His tag.
- Application
- Antibody Production (AbP), Standard (STD)
- Characteristics
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- Recombinant human PFKFB3 (full length, N-term HIS tag, transcript variant 1) protein expressed in E. coli.
- Produced with end-sequenced ORF clone
- Purity
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
- Top Product
- Discover our top product PFKFB3 Protein
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6-phosphofructo-2-Kinase/fructose-2,6-Biphosphatase 3 (PFKFB3) (AA 1-520) protein (GST tag)
PFKFB3 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
6-phosphofructo-2-Kinase/fructose-2,6-Biphosphatase 3 (PFKFB3) (AA 412-520) protein (GST tag)PFKFB3 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
6-phosphofructo-2-Kinase/fructose-2,6-Biphosphatase 3 (PFKFB3) protein (His tag)PFKFB3 Origin: Human Host: Escherichia coli (E. coli) Recombinant >95 % as determined by reducing SDS-PAGE.
6-phosphofructo-2-Kinase/fructose-2,6-Biphosphatase 3 (PFKFB3) protein (GST tag,His tag)PFKFB3 Origin: Human Host: Baculovirus infected Insect Cells Recombinant > 85 % as determined by reducing SDS-PAGE.
6-phosphofructo-2-Kinase/fructose-2,6-Biphosphatase 3 (PFKFB3) (AA 1-520) protein (Strep Tag)Crystallography grade PFKFB3 Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - Comment
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The tag is located at the N-terminal.
- Restrictions
- For Research Use only
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- Concentration
- 50 μg/mL
- Buffer
- 50 mM Tris,8M Urea, pH 8.0.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Target
- PFKFB3 (6-phosphofructo-2-Kinase/fructose-2,6-Biphosphatase 3 (PFKFB3))
- Alternative Name
- Pfkfb3 (PFKFB3 Products)
- Synonyms
- IPFK2 Protein, PFK2 Protein, E330010H22Rik Protein, iPFK-2 Protein, uPFK-2 Protein, PFKFB3 Protein, zgc:73257 Protein, wu:fc31e01 Protein, 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 Protein, 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 S homeolog Protein, PFKFB3 Protein, Pfkfb3 Protein, pfkfb3.S Protein, pfkfb3 Protein, PTRG_09894 Protein, Tsp_06710 Protein
- Background
- The protein encoded by this gene belongs to a family of bifunctional proteins that are involved in both the synthesis and degradation of fructose-2,6-bisphosphate, a regulatory molecule that controls glycolysis in eukaryotes. The encoded protein has a 6-phosphofructo-2-kinase activity that catalyzes the synthesis of fructose-2,6-bisphosphate (F2,6BP), and a fructose-2,6-biphosphatase activity that catalyzes the degradation of F2,6BP. This protein is required for cell cycle progression and prevention of apoptosis. It functions as a regulator of cyclin-dependent kinase 1, linking glucose metabolism to cell proliferation and survival in tumor cells. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene.
- Molecular Weight
- 59.4 kDa
- NCBI Accession
- NP_004557
- Pathways
- AMPK Signaling, Regulation of Carbohydrate Metabolic Process
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