POLH Protein (Myc-DYKDDDDK Tag)
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- Target See all POLH Proteins
- POLH (Polymerase (DNA Directed), eta (POLH))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This POLH protein is labelled with Myc-DYKDDDDK Tag.
- Application
- Antibody Production (AbP), Standard (STD)
- Characteristics
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- Recombinant human POLH / RAD30 protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
- Purity
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
- Top Product
- Discover our top product POLH Protein
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Polymerase (DNA Directed), eta (POLH) (AA 1-414) protein (GST tag)
POLH Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Polymerase (DNA Directed), eta (POLH) (AA 1-713) protein (His tag)Crystallography grade POLH Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, ELISA, Crys, SDS
Polymerase (DNA Directed), eta (POLH) (AA 1-694) protein (His tag)Crystallography grade POLH Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, ELISA, Crys, SDS
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - Comment
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The tag is located at the C-terminal.
- Restrictions
- For Research Use only
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- Concentration
- 50 μg/mL
- Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Target
- POLH (Polymerase (DNA Directed), eta (POLH))
- Alternative Name
- Polh,rad30 (POLH Products)
- Synonyms
- POLH Protein, fc50a12 Protein, wu:fc50a12 Protein, zgc:136881 Protein, zgc:152642 Protein, RAD30 Protein, RAD30A Protein, XP-V Protein, XPV Protein, polymerase (DNA directed), eta S homeolog Protein, DNA polymerase eta Protein, polymerase (DNA directed), eta Protein, putative DNA polymerase eta Protein, polymerase (DNA directed), eta (RAD 30 related) Protein, polh.S Protein, POLH Protein, polh Protein, Tb10.70.6190 Protein, LINJ_21_0690 Protein, Polh Protein, LOC100443019 Protein
- Background
- This gene encodes a member of the Y family of specialized DNA polymerases. It copies undamaged DNA with a lower fidelity than other DNA-directed polymerases. However, it accurately replicates UV-damaged DNA when thymine dimers are present, this polymerase inserts the complementary nucleotides in the newly synthesized DNA, thereby bypassing the lesion and suppressing the mutagenic effect of UV-induced DNA damage. This polymerase is thought to be involved in hypermutation during immunoglobulin class switch recombination. Mutations in this gene result in XPV, a variant type of xeroderma pigmentosum. Several transcript variants encoding different isoforms have been found for this gene.
- Molecular Weight
- 78.2 kDa
- NCBI Accession
- NP_006493
- Pathways
- DNA Damage Repair
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