PSMA1 Protein (His tag)
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- Target See all PSMA1 Proteins
- PSMA1 (Proteasome Subunit alpha Type 1 (PSMA1))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This PSMA1 protein is labelled with His tag.
- Application
- Antibody Production (AbP), Standard (STD)
- Characteristics
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- Recombinant human PSMA1 (full length, N-term HIS tag, transcript variant 2) protein expressed in E. coli.
- Produced with end-sequenced ORF clone
- Purity
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
- Top Product
- Discover our top product PSMA1 Protein
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Proteasome Subunit alpha Type 1 (PSMA1) (AA 1-263) protein (His tag)
PSMA1 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Proteasome Subunit alpha Type 1 (PSMA1) (AA 1-263) protein (GST tag)PSMA1 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Proteasome Subunit alpha Type 1 (PSMA1) (AA 1-263) protein (Strep Tag)Crystallography grade PSMA1 Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
Proteasome Subunit alpha Type 1 (PSMA1) (AA 1-263) protein (Strep Tag)Crystallography grade PSMA1 Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - Comment
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The tag is located at the N-terminal.
- Restrictions
- For Research Use only
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- Concentration
- 50 μg/mL
- Buffer
- 25 mM Tris, pH 8.0, 150 mM NaCl, 10 % glycerol. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Target
- PSMA1 (Proteasome Subunit alpha Type 1 (PSMA1))
- Alternative Name
- Psma1 (PSMA1 Products)
- Synonyms
- PSMA1 Protein, pros30 Protein, DDBDRAFT_0204226 Protein, DDBDRAFT_0214956 Protein, DDB_0204226 Protein, DDB_0214956 Protein, C2 Protein, HC2 Protein, Pros-30 Protein, alpha-type Protein, NU Protein, PROS30 Protein, CC2 Protein, zgc:92726 Protein, 143314_at Protein, Alpha-6 Protein, CG4904 Protein, Dmel\CG4904 Protein, PROS-35 Protein, PROS-Dm35 Protein, Pros-35 Protein, Pros-Dm35 Protein, Prosalpha6 Protein, alpha6 Protein, alpha_dm Protein, anon-SAGE:Wang-116 Protein, pros 35 Protein, pros35 Protein, proteasome subunit alpha 1 Protein, proteasome subunit alpha type 1 Protein, 20S proteasome subunit C2 Protein, proteasome (prosome, macropain) subunit, alpha type 1 Protein, proteasome subunit alpha 1 L homeolog Protein, Proteasome alpha6 subunit Protein, PSMA1 Protein, psma1 Protein, CNB05540 Protein, psmA1 Protein, Psma1 Protein, psma1.L Protein, Prosalpha6 Protein
- Background
- The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Jan 2009].
- Molecular Weight
- 28.9 kDa
- NCBI Accession
- NP_002777
- Pathways
- Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA
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