PTPN1 Protein (His tag)

Catalog No. ABIN2730106

Product Details

Target: PTPN1 (Protein tyrosine Phosphatase, Non-Receptor Type 1 (PTPN1))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This PTPN1 protein is labelled with His tag.

Application: Antibody Production (AbP), Standard (STD), Functional Studies (Func), Protein Interaction (PI)

Specificity: Optimal preservation of protein structure, post-translational modifications and functions.

Characteristics:

• Recombinant human PTPN1 protein expressed in E. coli.
• Produced with end-sequenced ORF clone
• Tested for bioactivity.

Purity: > 90 % as determined by SDS-PAGE and Coomassie blue staining

Biological Activity Comment: Specific activity: ~14000 nmol/min/mg. Enzymatic activity was determined by measuring the amount of 4-Nitrophenol hydrolyzed from p-nitrophenyl phosphate (pNPP) at 30C.

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays

Comment:

The tag is located at the N-terminal.

Restrictions: For Research Use only

Handling

Concentration: > 50 μg/mL

Buffer: 25 mM HEPES, 50 mM NaCl, 2.5 mM EDTA, PH7.5,5 mM DTT. < td valign=top>

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: PTPN1 (Protein tyrosine Phosphatase, Non-Receptor Type 1 (PTPN1))

Alternative Name: Ptpn1 (PTPN1 Products)

Synonyms: PTPN1 Protein, PTP1B Protein, PTP-1B Protein, PTP-HA2 Protein, Ptp Protein, ptp1b Protein, wu:fk54h03 Protein, CPTP1 Protein, protein tyrosine phosphatase, non-receptor type 1 Protein, protein tyrosine phosphatase, non-receptor type 1 L homeolog Protein, PTPN1 Protein, Ptpn1 Protein, ptpn1 Protein, ptpn1.L Protein

Background: The protein encoded by this gene is the founding member of the protein tyrosine phosphatase (PTP) family, which was isolated and identified based on its enzymatic activity and amino acid sequence. PTPs catalyze the hydrolysis of the phosphate monoesters specifically on tyrosine residues. Members of the PTP family share a highly conserved catalytic motif, which is essential for the catalytic activity. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP has been shown to act as a negative regulator of insulin signaling by dephosphorylating the phosphotryosine residues of insulin receptor kinase. This PTP was also reported to dephosphorylate epidermal growth factor receptor kinase, as well as JAK2 and TYK2 kinases, which implicated the role of this PTP in cell growth control, and cell response to interferon stimulation. Two transcript variants encoding different isoforms have been found for this gene.

Molecular Weight: 39.2 KDa

NCBI Accession: NP_002818

Pathways: TLR Signaling, Response to Growth Hormone Stimulus, ER-Nucleus Signaling, Platelet-derived growth Factor Receptor Signaling