PUMA Protein (Transcript Variant 1) (Myc-DYKDDDDK Tag)
(1 reference)-
- Target See all PUMA (BBC3) Proteins
- PUMA (BBC3) (BCL2 Binding Component 3 (BBC3))
- Protein Type
- Recombinant
- Protein Characteristics
- Transcript Variant 1
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This PUMA protein is labelled with Myc-DYKDDDDK Tag.
- Application
- Antibody Production (AbP), Standard (STD)
- Characteristics
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- Recombinant human PUMA (transcript variant 1) protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
- Purity
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
- Top Product
- Discover our top product BBC3 Protein
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BCL2 Binding Component 3 (BBC3) (AA 141-261) protein (His-IF2DI Tag)
BBC3 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % by SDS-PAGE gel analyses WB, ELISA
BCL2 Binding Component 3 (BBC3) (AA 1-193) protein (Strep Tag)Crystallography grade BBC3 Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
BCL2 Binding Component 3 (BBC3) (AA 1-261) protein (Strep Tag)Crystallography grade BBC3 Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
BCL2 Binding Component 3 (BBC3) (AA 1-193) protein (Strep Tag)Crystallography grade BBC3 Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
BCL2 Binding Component 3 (BBC3) (AA 76-170) protein (His tag,Strep II tag)BBC3 Origin: Human Host: Escherichia coli (E. coli) Recombinant ~95 % WB, ELISA, MS
BCL2 Binding Component 3 (BBC3) (Transcript Variant 4) protein (Myc-DYKDDDDK Tag)BBC3 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - Comment
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The tag is located at the C-terminal.
- Restrictions
- For Research Use only
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- Concentration
- 50 μg/mL
- Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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: "HER2 phosphorylates and destabilizes pro-apoptotic PUMA, leading to antagonized apoptosis in cancer cells." in: PLoS ONE, Vol. 8, Issue 11, pp. e78836, (2013) (PubMed).
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: "HER2 phosphorylates and destabilizes pro-apoptotic PUMA, leading to antagonized apoptosis in cancer cells." in: PLoS ONE, Vol. 8, Issue 11, pp. e78836, (2013) (PubMed).
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- Target
- PUMA (BBC3) (BCL2 Binding Component 3 (BBC3))
- Alternative Name
- Puma (BBC3 Products)
- Synonyms
- BBC3 Protein, Puma Protein, JFY-1 Protein, JFY1 Protein, PUMA Protein, PUMA/JFY1 Protein, BCL2 binding component 3 Protein, Bcl-2 binding component 3 Protein, BBC3 Protein, Bbc3 Protein, bbc3 Protein
- Background
- This gene encodes a member of the BCL-2 family of proteins. This family member belongs to the BH3-only pro-apoptotic subclass. The protein cooperates with direct activator proteins to induce mitochondrial outer membrane permeabilization and apoptosis. It can bind to anti-apoptotic Bcl-2 family members to induce mitochondrial dysfunction and caspase activation. Because of its pro-apoptotic role, this gene is a potential drug target for cancer therapy and for tissue injury. Alternative splicing results in multiple transcript variants.
- Molecular Weight
- 26.3 kDa
- NCBI Accession
- NP_001120712
- Pathways
- p53 Signaling, Positive Regulation of Endopeptidase Activity
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