RAD52 Protein (Myc-DYKDDDDK Tag)
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- Target See all RAD52 Proteins
- RAD52 (RAD52 Homolog (S. Cerevisiae) (RAD52))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This RAD52 protein is labelled with Myc-DYKDDDDK Tag.
- Application
- Antibody Production (AbP), Standard (STD)
- Characteristics
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- Recombinant human RAD52 protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
- Purity
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
- Top Product
- Discover our top product RAD52 Protein
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RAD52 Homolog (S. Cerevisiae) (RAD52) (AA 1-418) protein (His tag)
RAD52 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
RAD52 Homolog (S. Cerevisiae) (RAD52) (AA 1-418) protein (Strep Tag)Crystallography grade RAD52 Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, SDS, WB
RAD52 Homolog (S. Cerevisiae) (RAD52) (AA 1-420) protein (Strep Tag)Crystallography grade RAD52 Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, SDS, WB
RAD52 Homolog (S. Cerevisiae) (RAD52) (AA 60-282), (partial) protein (His-SUMO Tag)RAD52 Origin: Saccharomyces cerevisiae Host: Escherichia coli (E. coli) Recombinant > 90 % SDS
RAD52 Homolog (S. Cerevisiae) (RAD52) (Transcript Variant 3) protein (Myc-DYKDDDDK Tag)RAD52 Origin: Human Host: HEK-293T Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - Comment
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The tag is located at the C-terminal.
- Restrictions
- For Research Use only
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- Concentration
- 50 μg/mL
- Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Target
- RAD52 (RAD52 Homolog (S. Cerevisiae) (RAD52))
- Alternative Name
- Rad52 (RAD52 Products)
- Synonyms
- Rad52yh Protein, RAD52 Protein, zgc:112400 Protein, LOC100223085 Protein, MGC115453 Protein, RAD52 homolog, DNA repair protein Protein, RAD52 homolog (S. cerevisiae) Protein, RAD52 homolog, DNA repair protein S homeolog Protein, recombinase Protein, DNA repair and recombination protein, putative Protein, RAD52 protein Protein, RAD52 Protein, Rad52 Protein, rad52 Protein, rad52.S Protein
- Background
- The protein encoded by this gene shares similarity with Saccharomyces cerevisiae Rad52, a protein important for DNA double-strand break repair and homologous recombination. This gene product was shown to bind single-stranded DNA ends, and mediate the DNA-DNA interaction necessary for the annealing of complementary DNA strands. It was also found to interact with DNA recombination protein RAD51, which suggested its role in RAD51 related DNA recombination and repair. A pseudogene of this gene is present on chromosome 2. Alternative splicing results in multiple transcript variants. Additional alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known.
- Molecular Weight
- 46 kDa
- NCBI Accession
- NP_602296
- Pathways
- DNA Damage Repair
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