R-Spondin 3 Protein (RSPO3)
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- Target See all R-Spondin 3 (RSPO3) Proteins
- R-Spondin 3 (RSPO3)
- Protein Type
- Recombinant
- Biological Activity
- Active
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Origin
- Human
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Source
- CHO Cells
- Application
- Antibody Production (AbP), Standard (STD), Functional Studies (Func), Protein Interaction (PI)
- Specificity
- Optimal preservation of protein structure, post-translational modifications and functions.
- Characteristics
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- Recombinant human RSPO3 protein expressed in CHO cells.
- Produced with end-sequenced ORF clone
- Tested for bioactivity.
- Purity
- > 95 % as determined by SDS-PAGE and Coomassie blue staining
- Endotoxin Level
- Endotoxin level is <0.1 ng/μg of protein (<1EU/μg).
- Biological Activity Comment
- R-Spondin-3 enhances BMP-2 mediated differentiation of MC3T3-E1 cells.
- Top Product
- Discover our top product RSPO3 Protein
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R-Spondin 3 (RSPO3) (AA 24-272) protein (His-IF2DI Tag)
RSPO3 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % by SDS-PAGE gel analyses WB, ELISA
R-Spondin 3 (RSPO3) (AA 22-272) protein (His tag)Crystallography grade RSPO3 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
R-Spondin 3 (RSPO3) (AA 22-277) protein (His tag)Crystallography grade RSPO3 Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays - Restrictions
- For Research Use only
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- Buffer
- Lyophilized from a 0.2 μM filtered solution of 20 mM phosphate buffer,100 mM NaCl, pH 7.2
- Handling Advice
- Resuspend the protein in the desired concentration in proper buffer
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Target
- R-Spondin 3 (RSPO3)
- Alternative Name
- Rspo3 (RSPO3 Products)
- Synonyms
- pwtsr Protein, thsd2 Protein, cristin1 Protein, CRISTIN1 Protein, PWTSR Protein, THSD2 Protein, RGD1563246 Protein, 2810459H04Rik Protein, AW742308 Protein, Cristin1 Protein, Thsd2 Protein, R-spondin 3 Protein, rspo3 Protein, RSPO3 Protein, Rspo3 Protein
- Background
- This gene belongs to the R-spondin family. The encoded protein plays a role in the regulation of Wnt (wingless-type MMTV integration site family)/beta-catenin and Wnt/planar cell polarity (PCP) signaling pathways, which are involved in development, cell growth and disease pathogenesis. Genome-wide association studies suggest a correlation of this gene with bone mineral density and risk of fracture. This gene may be involved in tumor development.
- Molecular Weight
- 26.9 kDa
- NCBI Accession
- NP_116173
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