RUNX2 Protein (His tag)

Catalog No. ABIN2731252

Product Details

Target: RUNX2 (Runt-Related Transcription Factor 2 (RUNX2))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This RUNX2 protein is labelled with His tag.

Application: Antibody Production (AbP), Standard (STD)

Characteristics:

• Recombinant human Runt-related transcription factor 2 (RUNX2), transcript variant 3, full length, with N-terminal HIS tag, expressed in E.Coli, 50 μg (full length, N-term HIS tag, transcript variant 3) protein expressed in E. coli.
• Produced with end-sequenced ORF clone

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the N-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris, pH 8.0, 150 mM NaCl, 10 % glycerol, 1 % Sarkosyl. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: RUNX2 (Runt-Related Transcription Factor 2 (RUNX2))

Abstract: RUNX2 Products

Synonyms: AML3 Protein, CBF-alpha-1 Protein, CBFA1 Protein, CCD Protein, CCD1 Protein, CLCD Protein, OSF-2 Protein, OSF2 Protein, PEA2aA Protein, PEBP2aA Protein, Cbf Protein, Cbfa-1 Protein, Cbfa1 Protein, LS3 Protein, Osf2 Protein, Pebp2a1 Protein, Pebpa2a Protein, runx2 Protein, RUNX2 Protein, ccd Protein, aml3 Protein, ccd1 Protein, osf2 Protein, cbfa1 Protein, pea2aa Protein, pebp2a1 Protein, pebp2a2 Protein, pebp2aa Protein, pebp2aa1 Protein, runt related transcription factor 2 Protein, runt-related transcription factor 2a Protein, runt-related transcription factor 2 Protein, runt related transcription factor 2 L homeolog Protein, RUNX2 Protein, runx2a Protein, Runx2 Protein, runx2 Protein, LOC703331 Protein, LOC100549663 Protein, runx2.L Protein

Background: This gene is a member of the RUNX family of transcription factors and encodes a nuclear protein with an Runt DNA-binding domain. This protein is essential for osteoblastic differentiation and skeletal morphogenesis and acts as a scaffold for nucleic acids and regulatory factors involved in skeletal gene expression. The protein can bind DNA both as a monomer or, with more affinity, as a subunit of a heterodimeric complex. Two regions of potential trinucleotide repeat expansions are present in the N-terminal region of the encoded protein, and these and other mutations in this gene have been associated with the bone development disorder cleidocranial dysplasia (CCD). Transcript variants that encode different protein isoforms result from the use of alternate promoters as well as alternate splicing.

Molecular Weight: 54.9 kDa

NCBI Accession: NP_004339