RUNX1 Protein (Transcript Variant 2) (Myc-DYKDDDDK Tag)

Catalog No. ABIN2731253

Product Details

Target: RUNX1 (Runt-Related Transcription Factor 1 (RUNX1))

Protein Type: Recombinant

Biological Activity: Active

Protein Characteristics: Transcript Variant 2

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This RUNX1 protein is labelled with Myc-DYKDDDDK Tag.

Application: Antibody Production (AbP), Functional Studies (Func), Protein Interaction (PI), Standard (STD)

Specificity: Optimal preservation of protein structure, post-translational modifications and functions.

Characteristics:

• Recombinant human RUNX1 (transcript variant 2) protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone
• Tested for bioactivity.

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Biological Activity Comment: RUNX1 Activity verified in a DNA-binding assay:

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: > 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

References

Mochin, Underwood, Cooper, McLenithan, Pierce, Nalvarte, Arbiser, Karlsson, Moise, Moskovitz, Passaniti: "Hyperglycemia and redox status regulate RUNX2 DNA-binding and an angiogenic phenotype in endothelial cells." in: Microvascular research, Vol. 97, pp. 55-64, (2014) (PubMed).

Target Details

Target: RUNX1 (Runt-Related Transcription Factor 1 (RUNX1))

Alternative Name: Runx1 (RUNX1 Products)

Synonyms: RUNX1 Protein, runx1 Protein, AI462102 Protein, AML1 Protein, Cbfa2 Protein, Pebp2a2 Protein, Pebpa2b Protein, AML1-EVI-1 Protein, AMLCR1 Protein, CBFA2 Protein, EVI-1 Protein, PEBP2aB Protein, runxa Protein, Runx-1 Protein, XAML Protein, Xaml1 Protein, aml Protein, aml-1 Protein, aml1 Protein, aml1-evi-1 Protein, amlcr1 Protein, cbfa2 Protein, evi-1 Protein, pebp2ab Protein, Aml1 Protein, uncharacterized LOC473981 Protein, runt-related transcription factor Protein, runt related transcription factor 1 Protein, runt-related transcription factor 1 Protein, runt related transcription factor 1 L homeolog Protein, LOC473981 Protein, runt Protein, Runx1 Protein, RUNX1 Protein, runx1 Protein, runx1.L Protein

Background: Core binding factor (CBF) is a heterodimeric transcription factor that binds to the core element of many enhancers and promoters. The protein encoded by this gene represents the alpha subunit of CBF and is thought to be involved in the development of normal hematopoiesis. Chromosomal translocations involving this gene are well-documented and have been associated with several types of leukemia. Three transcript variants encoding different isoforms have been found for this gene.

Molecular Weight: 48.6 kDa

NCBI Accession: NP_001001890