SAE1 Protein (Transcript Variant 1) (Myc-DYKDDDDK Tag)
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- Target See all SAE1 Proteins
- SAE1 (SUMO1 Activating Enzyme Subunit 1 (SAE1))
- Protein Type
- Recombinant
- Protein Characteristics
- Transcript Variant 1
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This SAE1 protein is labelled with Myc-DYKDDDDK Tag.
- Application
- Antibody Production (AbP), Standard (STD)
- Characteristics
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- Recombinant human SAE1 / AOS1 (transcript variant 1) protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
- Purity
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
- Top Product
- Discover our top product SAE1 Protein
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SUMO1 Activating Enzyme Subunit 1 (SAE1) (AA 1-346) protein (His tag)
SAE1 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
SUMO1 Activating Enzyme Subunit 1 (SAE1) (AA 1-346) protein (GST tag)SAE1 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
SUMO1 Activating Enzyme Subunit 1 (SAE1) (AA 1-346) protein (Strep Tag)Crystallography grade SAE1 Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
SUMO1 Activating Enzyme Subunit 1 (SAE1) (AA 2-346) protein (His tag)Crystallography grade SAE1 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS, Crys
SUMO1 Activating Enzyme Subunit 1 (SAE1) (AA 1-350) protein (Strep Tag)Crystallography grade SAE1 Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
SUMO1 Activating Enzyme Subunit 1 (SAE1) (AA 2-350) protein (His tag)Crystallography grade SAE1 Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS, Crys
SUMO1 Activating Enzyme Subunit 1 (SAE1) (Transcript Variant 2) protein (Myc-DYKDDDDK Tag)SAE1 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - Comment
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The tag is located at the C-terminal.
- Restrictions
- For Research Use only
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- Concentration
- 50 μg/mL
- Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Target
- SAE1 (SUMO1 Activating Enzyme Subunit 1 (SAE1))
- Alternative Name
- Sae1,aos1 (SAE1 Products)
- Synonyms
- AOS1 Protein, HSPC140 Protein, SUA1 Protein, UBLE1A Protein, 2400010M20Rik Protein, 2610044L12Rik Protein, AL033372 Protein, AW743391 Protein, D7Ertd177e Protein, Sua1 Protein, Uble1a Protein, Aos1p Protein, Sua1p Protein, aos Protein, sae2a Protein, uble1a Protein, wu:fa28b04 Protein, zgc:86633 Protein, SUMO1 activating enzyme subunit 1 Protein, SUMO1 activating enzyme subunit 1 L homeolog Protein, SAE1 Protein, Sae1 Protein, sae1.L Protein, sae1 Protein
- Background
- Posttranslational modification of proteins by the addition of the small protein SUMO (see SUMO1 MIM 601912), or sumoylation, regulates protein structure and intracellular localization. SAE1 and UBA2 (MIM 613295) form a heterodimer that functions as a SUMO-activating enzyme for the sumoylation of proteins (Okuma et al., 1999 [PubMed 9920803]).[supplied by OMIM, Mar 2010].
- Molecular Weight
- 38.3 kDa
- NCBI Accession
- NP_005491
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