SAA1 Protein (Transcript Variant 1)

Catalog No. ABIN2731848

Product Details

Target: SAA1 (Serum Amyloid A1 (SAA1))

Protein Type: Recombinant

Biological Activity: Active

Protein Characteristics: Transcript Variant 1

Origin: Human

Source: Escherichia coli (E. coli)

Application: Functional Studies (Func), Antibody Production (AbP), Standard (STD), Protein Interaction (PI)

Specificity: Optimal preservation of protein structure, post-translational modifications and functions.

Characteristics:

• Recombinant human Serum amyloid A1 (SAA1), transcript variant 1, with substitutions of asparagine for aspartic acid at position 60 and arginine for histidine at position 71. (transcript variant 1) protein expressed in E. coli.
• Produced with end-sequenced ORF clone
• Tested for bioactivity.

Purity: > 95 % as determined by SDS-PAGE and Coomassie blue staining

Endotoxin Level: Endotoxin level is <0.1 ng/μg of protein (<1EU/μg).

Biological Activity Comment: Determined by its ability to chemoattract human monocytes using a concentration range of 10.0-100.0 ng/ml.

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays

Restrictions: For Research Use only

Handling

Buffer: Lyophilized from a 0.2 μM filtered solution of 20 mM phosphate buffer,100 mM NaCl, pH 7.2

Handling Advice: Resuspend the protein in the desired concentration in proper buffer

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: SAA1 (Serum Amyloid A1 (SAA1))

Abstract: SAA1 Products

Synonyms: PIG4 Protein, SAA Protein, SAA2 Protein, TP53I4 Protein, zgc:103580 Protein, SAA1 Protein, Saa-1 Protein, Saa2 Protein, serum amyloid A1 Protein, serum amyloid A Protein, amyloid protein A Protein, serum amyloid A protein Protein, serum amyloid a protein Protein, serum amyloid A 1 Protein, serum amyloid protein A Protein, SAA1 Protein, saa Protein, LOC694827 Protein, Sden_2438 Protein, Mmc1_0571 Protein, Riean_0971 Protein, Saa1 Protein, LOC100009168 Protein, LOC101120204 Protein

Background: This gene encodes a member of the serum amyloid A family of apolipoproteins. The encoded preproprotein is proteolytically processed to generate the mature protein. This protein is a major acute phase protein that is highly expressed in response to inflammation and tissue injury. This protein also plays an important role in HDL metabolism and cholesterol homeostasis. High levels of this protein are associated with chronic inflammatory diseases including atherosclerosis, rheumatoid arthritis, Alzheimer's disease and Crohn's disease. This protein may also be a potential biomarker for certain tumors. Alternate splicing results in multiple transcript variants that encode the same protein. A pseudogene of this gene is found on chromosome 11.

Molecular Weight: 11.7 kDa

NCBI Accession: NP_000322

Pathways: Toll-Like Receptors Cascades